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Sensitive and Specific Detection of Ewing Sarcoma Minimal Residual Disease in Ovarian and Testicular Tissues in an In Vitro Model
- Source :
- HAL, ESHRE, ESHRE, 2018, Barcelone, Spain, Collège de Médecine de la Reproduction, Collège de Médecine de la Reproduction, 2017, Paris, France, CNRC, CNRC, 2017, Lille, France, Cancers, Cancers, MDPI, 2019, 11, pp.1807. ⟨10.3390/cancers11111807⟩, Oncauvergnales, Oncauvergnales, 2017, Clermont-Ferrand, France, Cancers, Vol 11, Iss 11, p 1807 (2019), BLEFCO CECOS, BLEFCO CECOS, 2020, Paris, France, Volume 11, Issue 11, BLEFCO CECOS, 2019, Paris, France, FFER, FFER, 2017, Tour, France, Cancers, 2019, 11, pp.1807. ⟨10.3390/cancers11111807⟩
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Abstract
- Ewing sarcoma (EWS) is a common pediatric solid tumor with high metastatic potential. Due to toxic effects of treatments on reproductive functions, the cryopreservation of ovarian tissue (OT) or testicular tissue (TT) is recommended to preserve fertility. However, the risk of reintroducing residual metastatic tumor cells should be evaluated before fertility restoration. Our goal was to validate a sensitive and specific approach for EWS minimal residual disease (MRD) detection in frozen germinal tissues. Thawed OT (n = 12) and TT (n = 14) were contaminated with tumor RD-ES cells (10, 100, and 1000 cells) and EWS-FLI1 tumor-specific transcript was quantified with RT-qPCR. All contaminated samples were found to be positive, with a strong correlation between RD-ES cell numbers and EWS-FLI1 levels in OT (r = 0.93) and TT (r = 0.96) (p &lt<br />0.001). No transcript was detected in uncontaminated control samples. The invasive potential of Ewing cells was evaluated using co-culture techniques. After co-culturing, tumor cells were detected in OT/TT with histology, FISH, and RT-qPCR. In addition, four OT and four TT samples from children with metastatic EWS were tested, and no MRD was found using RT-qPCR and histology. We demonstrated the high sensitivity and specificity of RT-qPCR to detect EWS MRD in OT/TT samples. Clinical trial: NCT 02400970.
- Subjects :
- Cancer Research
[SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Testicular tissue
fertility preservation
[SDV]Life Sciences [q-bio]
Cell
testicular tissue
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.GEN] Life Sciences [q-bio]/Genetics
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
lcsh:RC254-282
Cryopreservation
Article
minimal residual disease detection
03 medical and health sciences
0302 clinical medicine
[SDV.BDD] Life Sciences [q-bio]/Development Biology
medicine
[SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Fertility preservation
[SDV.BDD]Life Sciences [q-bio]/Development Biology
[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology
ovarian tissue
ComputingMilieux_MISCELLANEOUS
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics
[SDV.GEN]Life Sciences [q-bio]/Genetics
030219 obstetrics & reproductive medicine
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
business.industry
Ovarian tissue
RT-qPCR
Histology
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Minimal residual disease
3. Good health
[SDV] Life Sciences [q-bio]
[SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Sarcoma
business
Ewing sarcoma
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- ISSN :
- 20726694
- Database :
- OpenAIRE
- Journal :
- HAL, ESHRE, ESHRE, 2018, Barcelone, Spain, Collège de Médecine de la Reproduction, Collège de Médecine de la Reproduction, 2017, Paris, France, CNRC, CNRC, 2017, Lille, France, Cancers, Cancers, MDPI, 2019, 11, pp.1807. ⟨10.3390/cancers11111807⟩, Oncauvergnales, Oncauvergnales, 2017, Clermont-Ferrand, France, Cancers, Vol 11, Iss 11, p 1807 (2019), BLEFCO CECOS, BLEFCO CECOS, 2020, Paris, France, Volume 11, Issue 11, BLEFCO CECOS, 2019, Paris, France, FFER, FFER, 2017, Tour, France, Cancers, 2019, 11, pp.1807. ⟨10.3390/cancers11111807⟩
- Accession number :
- edsair.doi.dedup.....e115db859e2ade5d75f7bcb2501aa73b