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Functional and topographical analyses of epitopes on bovine herpesvirus type 1 glycoprotein IV
- Source :
- Archives of Virology
- Publication Year :
- 1988
- Publisher :
- Springer Science and Business Media LLC, 1988.
-
Abstract
- Summary Bovine herpesvirus type 1 (BHV-1) glycoprotein gIV was purified by affinity chromatography. Purified preparations showed two distinct components of 71 K and 140 K following electrophoresis in sodium dodecyl sulphate polyacrylamide gels. The polypeptides were separated, excised from the gel and used to immunize rabbits; the resulting antisera showed a high degree of cross reactivity indicating that these polypeptides represent monomeric and dimeric forms of the same glycoprotein. Purified gIV was also used to develop a gIV-specific panel of monoclonal antibodies. Neutralizing monoclonal antibodies directed against gIV were conjugated to horseradish peroxidase and subjected to competition binding assays by ELISA. Three distinct neutralizing antigenic domains on gIV were identified. Domain 1 comprised two overlapping epitopes, whereas domain 2 was represented by a single monoclonal antibody. The third antigenic domain was made up of a complex of four identical or overlapping epitopes designated 3a, b, c, and d. Evidence is presented suggesting that domain 1 of gIV may be involved in penetration of the virus into the cell.
- Subjects :
- Virus Cultivation
Polyacrylamide
medicine.drug_class
Monoclonal Antibody
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
medicine.disease_cause
Binding, Competitive
Cross-reactivity
Chromatography, Affinity
Epitope
Epitopes
Viral Proteins
Affinity chromatography
Antigen
Neutralization Tests
Sodium Dodecyl
Virology
medicine
Antigens, Viral
Herpesvirus 1, Bovine
chemistry.chemical_classification
Antiserum
biology
Antibodies, Monoclonal
General Medicine
biology.organism_classification
Original Papers
Molecular biology
Bovine herpesvirus 1
Molecular Weight
chemistry
Dodecyl
Electrophoresis, Polyacrylamide Gel
Polypeptide
Glycoprotein
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....e105074449cbac900649ae570b3cc38c