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Identification of influenza C virus phosphoproteins
- Source :
- Microbiology and immunology. 39(9)
- Publication Year :
- 1995
-
Abstract
- The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP), and matrix protein (M1) are all phosphorylated in both infected cells and virions. It was also observed that, in the presence of trypsin (10 micrograms/ml), the unphosphorylated form of the HE glycoprotein was cleaved efficiently whereas the phosphorylated form was not, raising the possibility that phosphorylation of HE may influence its susceptibility to degradation by proteolytic enzymes.
- Subjects :
- Radioimmunoprecipitation Assay
Influenzavirus C
Immunology
Orthomyxoviridae
Hemagglutinins, Viral
Microbiology
Virus
Phosphates
Viral Matrix Proteins
Viral Proteins
Virology
medicine
Tumor Cells, Cultured
Humans
Phosphorylation
chemistry.chemical_classification
Viral matrix protein
biology
Proteolytic enzymes
Antibodies, Monoclonal
biology.organism_classification
Trypsin
Phosphoproteins
Molecular biology
Nucleoprotein
Nucleoproteins
Biochemistry
chemistry
Electrophoresis, Polyacrylamide Gel
Influenza C Virus
Glycoprotein
Viral Fusion Proteins
medicine.drug
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 39
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Microbiology and immunology
- Accession number :
- edsair.doi.dedup.....e0fd54b4d0713c57f30290555cde9c13