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European multicenter evaluation of Xpert® Xpress SARS‐CoV‐2/Flu/RSV test
- Source :
- Journal of Medical Virology, Journal of Medical Virology, 93, 10, pp. 5798-5804, Journal of Medical Virology, 93, 5798-5804
- Publication Year :
- 2021
- Publisher :
- John Wiley and Sons Inc., 2021.
-
Abstract
- Contains fulltext : 238214.pdf (Publisher’s version ) (Open Access) Rapid diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are paramount for reducing the spread of the current pandemic. During additional seasonal epidemics with influenza A/B and respiratory syncytial virus (RSV), the clinical signs and symptoms cannot be distinguished easily from SARS-CoV-2. Therefore, a new assay combining four targets in the form of the new Xpert Xpress SARS-CoV-2/Flu/RSV assay was evaluated. The assay was compared to the Xpert Xpress SARS-CoV-2, Xpert Xpress Flu/RSV, Seegene Flu/RSV, influenza A/B r-gene® and RSV/hMPV r-gene®. A total of 295 nasopharyngeal and throat swabs were tested at four institutes throughout Europe including 72 samples positive for SARS-CoV-2, 65 for influenza A, 47 for influenza B, and 77 for RSV. The sensitivity of the new assay was above 95% for all targets, with the highest for SARS-CoV-2 (97.2%). The overall correlation of SARS-CoV-2 Ct values between Xpert Xpress SARS-CoV-2 assay and Xpert Xpress SARS-CoV-2/Flu/RSV assay was high. The agreement between Ct values above 30 showed the multiplex giving higher Ct values for SARS-CoV-2 on average than the singleplex assay. In conclusion, the new assay is a rapid and reliable alternative with less hands-on time for the detection of not one, but four upper respiratory tract pathogens that may circulate at the same time.
- Subjects :
- 10028 Institute of Medical Virology
viruses
respiratory syncytial virus
medicine.disease_cause
SARS‐CoV‐2
0302 clinical medicine
Nasopharynx
Pandemic
540 Chemistry
Influenza A virus
Medicine
Multiplex
030212 general & internal medicine
skin and connective tissue diseases
Respiratory Tract Infections
Research Articles
10038 Institute of Clinical Chemistry
Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]
Respiratory tract infections
virus diseases
respiratory system
Europe
Infectious Diseases
Molecular Diagnostic Techniques
COVID-19 Nucleic Acid Testing
030211 gastroenterology & hepatology
influenza
Research Article
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Signs and symptoms
610 Medicine & health
Respiratory Syncytial Virus Infections
Sensitivity and Specificity
Virus
03 medical and health sciences
COVID‐19
Virology
Multiplex polymerase chain reaction
Influenza, Human
Humans
business.industry
SARS-CoV-2
pandemic
COVID-19
2725 Infectious Diseases
multiplex PCR
respiratory tract diseases
Influenza B virus
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Respiratory Syncytial Virus, Human
2406 Virology
business
Multiplex Polymerase Chain Reaction
Subjects
Details
- Language :
- English
- ISSN :
- 10969071, 01466615, and 57985804
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi.dedup.....e0e74804c0b1001720332c95fdef1afc