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Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase
- Source :
- Current Genetics. 20:53-61
- Publication Year :
- 1991
- Publisher :
- Springer Science and Business Media LLC, 1991.
-
Abstract
- The temperature-conditional pet mutant, ts379, of Saccharomyces cerevisiae fails to synthesize mitochondrial ATP synthase subunit 9 at the restrictive temperature due to mutation of a single nuclear locus, AEP2. The inability to synthesize subunit 9 correlates with a lowered accumulation of the cognate oli1 mRNA indicating that the AEP2 product is involved in oli1 transcript maturation or stabilization. The AEP2 gene has been isolated in this study from a wild-type yeast genomic library by genetic complementation of ts379 at the restrictive temperature. A 1,740 nucleotide open-reading frame was observed that encodes a basic, hydrophilic protein of 67,534 Da which possesses a putative mitochondrial address signal. Disruption of chromosomal DNA within this reading frame produced a non-conditional respiratory mutant unable to synthesize subunit 9, identifying the AEP2 gene. Hybridization analyses indicate that AEP2 is located on chromosome XIII and produces a 2.1 kb poly(A)+ transcript. Two additional open-reading frames were found in close proximity to that of AEP2. The three open-reading frames shared no significant homology with entries in several data bases.
- Subjects :
- Transcription, Genetic
RNA, Mitochondrial
Protein subunit
Genes, Fungal
Molecular Sequence Data
Restriction Mapping
Saccharomyces cerevisiae
Mutant
Mitochondrion
DNA, Mitochondrial
Gene Expression Regulation, Enzymologic
Fungal Proteins
Gene Expression Regulation, Fungal
Gene expression
Genetics
Genomic library
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
DNA, Fungal
Gene
Base Sequence
biology
ATP synthase
Genetic Complementation Test
RNA, Fungal
General Medicine
Blotting, Northern
biology.organism_classification
Molecular biology
Proton-Translocating ATPases
biology.protein
RNA
Subjects
Details
- ISSN :
- 14320983 and 01728083
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Current Genetics
- Accession number :
- edsair.doi.dedup.....e0d350e951f3117b9d2241ac6df0f5d1