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GRDN-1/Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans
- Source :
- Dev Biol
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Primary cilia are located at the dendritic tips of sensory neurons and house the molecular machinery necessary for detection and transduction of sensory stimuli. The mechanisms that coordinate dendrite extension with cilium position during sensory neuron development are not well understood. Here, we show that GRDN-1, the Caenorhabditis elegans ortholog of the highly conserved scaffold and signaling protein Girdin/GIV, regulates both cilium position and dendrite extension in the postembryonic AQR and PQR gas-sensing neurons. Mutations in grdn-1 disrupt dendrite outgrowth and mislocalize cilia to the soma or proximal axonal segments in AQR, and to a lesser extent, in PQR. GRDN-1 is localized to the basal body and regulates localization of HMR-1/Cadherin to the distal AQR dendrite. However, knockdown of HMR-1 and/or loss of SAX-7/LICAM, molecules previously implicated in sensory dendrite development in C. elegans, do not alter AQR dendrite morphology or cilium position. We find that GRDN-1 localization in AQR is regulated by UNC-116/Kinesin-1, and that correspondingly, unc-116 mutants exhibit severe AQR dendrite outgrowth and cilium positioning defects. In contrast, GRDN-1 and cilium localization in PQR is modulated by LIN-44/Wnt signaling. Together, these findings identify upstream regulators of GRDN-1, and describe new cell-specific roles for this multifunctional protein in sensory neuron development.
- Subjects :
- Sensory Receptor Cells
Neurogenesis
Kinesins
Cell Cycle Proteins
Dendrite
Biology
Article
Animals, Genetically Modified
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Basal body
Cilia
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Wnt Signaling Pathway
Molecular Biology
Glycoproteins
030304 developmental biology
0303 health sciences
Cilium
Microfilament Proteins
Dendrites
Cell Biology
Cadherins
Axons
Basal Bodies
Sensory neuron
Cell biology
Dendrite morphogenesis
medicine.anatomical_structure
Gene Knockdown Techniques
Mutation
Sensory dendrite
Dendrite extension
CRISPR-Cas Systems
Transduction (physiology)
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 472
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....e0d261f7e1af9a25c9ec42b1a56215a1