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Correlation of Developmental Differences of Nuclear Transfer Embryos Cells to the Methylation Profiles of Nuclear Transfer Donor Cells in Swine
- Source :
- ResearcherID
- Publication Year :
- 2007
- Publisher :
- Informa UK Limited, 2007.
-
Abstract
- Methylation of DNA is the most commonly studied epigenetic mechanism of developmental competence and somatic cell nuclear transfer (SCNT). Previous studies of epigenetics and the SCNT procedures have examined the effects of different culture media on donor cells and reconstructed embryos, and the methylation status of specific genes in the fetus or live offspring. Here we used a microarray based approach to identify the methylation profiles of SCNT donor cells including three clonal porcine fetal fibroblast-like cell sublines and adult somatic cells selected from kidney and mammary tissues. The methylation profiles of the donor cells were then analyzed with respect to their ability to direct development to the blastocyst stage after nuclear transfer. Clonal cell lines A2, A7 and A8 had blastocyst rates of 11.7%(a), 16.7%(ab) and 20.0%(b), respectively ((ab) p0.05). Adult somatic cells included kidney, mammary (large), and mammary (small) also had different blastocyst rates (ab p0.05) of 4.2% (a), 10.7% (ab) and 18.3% (b), respectively. For clonal donor cells and for adult somatic cell groups the donor cells with the highest blastocyst rates also had methylation profiles with the lowest similarity to the methylation profiles of the in vivo-produced blastocysts. Conversely, the donor cells with the lowest blastocyst rates had methylation profiles with the highest similarity to the methylation profiles of the in vivo-produced blastocysts. Our findings show there is an inverse correlation to the similarity of the methylation profiles of the donor cells and the in vivo-produced embryos, and to the blastocyst rates following SCNT.
- Subjects :
- Male
Nuclear Transfer Techniques
Cancer Research
Swine
Somatic cell
Cloning, Organism
Biology
Kidney
Article
Epigenesis, Genetic
Mammary Glands, Animal
medicine
Animals
Epigenetics
Blastocyst
Molecular Biology
Cells, Cultured
Cell Nucleus
Embryo
Methylation
DNA Methylation
Fibroblasts
Microarray Analysis
Molecular biology
medicine.anatomical_structure
Cell culture
embryonic structures
DNA methylation
Somatic cell nuclear transfer
Female
Subjects
Details
- ISSN :
- 15592308 and 15592294
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Epigenetics
- Accession number :
- edsair.doi.dedup.....e0bc36fccc1c3ffb55b2e58459552135