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Effects of inhaled corticosteroids on the expression of TNF family molecules in murine model of allergic asthma
- Source :
- Experimental lung research. 43(8)
- Publication Year :
- 2017
-
Abstract
- Background: The tumor necrosis factor superfamily member LIGHT (the official gene symbol approved by NCBI Gene Database), an inflammatory factor secreted by T cells after allergen exposure, recently discovered to play crucial roles in asthmatic airway remodeling. However, it is unclear whether LIGHT could be controlled by inhaled corticosteroids, a key component of asthma management. This study was to investigate the effects and potential mechanisms of inhaled budesonide on the expressions of LIGHT and its receptors (LTβR and HVEM) of lung tissues in ovalbumin-sensitized mice. Materials and Methods: Thirty-three BALB/c mice were randomly divided into the control, asthma model, and budesonide treatment groups (11 in each group). Mice were sensitized and challenged by OVA to develop mouse model of chronic asthma, and treated with aerosolized budesonide before OVA challenge. Bronchoalveolar lavage fluid (BALF) and lungs were obtained after the final OVA challenge. Protein and mRNA Levels of LIGHT, LTβR, and HVEM in the lungs were investigated by immunohistochemistry, image analysis, and real-time PCR. Expressions of IL-6 and IFN-γ in BALF were measured by ELISA. Results: Inhaled budesonide significantly reduced protein and mRNA levels of lung LIGHT, LTβR, and HVEM in asthmatic mice. Correspondingly, the number of eosinophils and neutrophils and IL-6 levels in BALF after budesonide treatment were found to be decreased, whereas the IFN-γ levels in BALF were increased. Moreover, the expressions of LIGHT and HVEM mRNA showed positive correlation with IL-6 levels in the treatment group. Conclusions: Inhaled budesonide can down-regulate the expressions of LIGHT, LTβR, and HVEM in the lungs of asthmatic mice, and LIGHT/LTβR/HVEM interactions may be a potentially key target for asthma treatment.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Budesonide
Tumor Necrosis Factor Ligand Superfamily Member 14
Clinical Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Adrenal Cortex Hormones
Lymphotoxin beta Receptor
medicine
Animals
Receptor
Molecular Biology
Asthma
Lung
medicine.diagnostic_test
business.industry
respiratory system
medicine.disease
respiratory tract diseases
030104 developmental biology
Bronchoalveolar lavage
medicine.anatomical_structure
030228 respiratory system
Asthmatic Airway Remodeling
Immunology
Tumor Necrosis Factors
Immunohistochemistry
Tumor necrosis factor alpha
business
Receptors, Tumor Necrosis Factor, Member 14
medicine.drug
Subjects
Details
- ISSN :
- 15210499
- Volume :
- 43
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Experimental lung research
- Accession number :
- edsair.doi.dedup.....e0a45497515da99c448e3a05040a2da6