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Symmetrical organization of proteins under docked synaptic vesicles
- Source :
- Febs Letters
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- During calcium-regulated exocytosis, the constitutive fusion machinery is 'clamped' in a partially assembled state until synchronously released by calcium. The protein machinery involved in this process is known, but the supra-molecular architecture and underlying mechanisms are unclear. Here, we use cryo-electron tomography analysis in nerve growth factor-differentiated neuro-endocrine (PC12) cells to delineate the organization of the release machinery under the docked vesicles. We find that exactly six exocytosis modules, each likely consisting of a single SNAREpin with its bound Synaptotagmins, Complexin, and Munc18 proteins, are symmetrically arranged at the vesicle-PM interface. Mutational analysis suggests that the symmetrical organization is templated by circular oligomers of Synaptotagmin. The observed arrangement, including its precise radial positioning, is in-line with the recently proposed 'buttressed ring hypothesis'.
- Subjects :
- Electron Microscope Tomography
Munc18 Proteins
SNARE proteins
Biophysics
Nerve Tissue Proteins
Biochemistry
Synaptic vesicle
Exocytosis
Synaptotagmin 1
Synaptotagmins
03 medical and health sciences
Complexin
Structural Biology
Nerve Growth Factor
Research Letter
Neurites
Genetics
Animals
Structural Biology. Membrane trafficking, vesicles, organelles
Molecular Biology
030304 developmental biology
regulated exocytosis
0303 health sciences
cryo‐electron tomography
Chemistry
Vesicle
Cryoelectron Microscopy
030302 biochemistry & molecular biology
PC12 cells
Cell Biology
Research Letters
Rats
synaptotagmin
Mutation
Cryo-electron tomography
Calcium
Synaptic Vesicles
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 593
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....e099ff82bea513329c5039d8c11cc1f7