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Expression and characterization of Mycobacterium tuberculosis CYP144: Common themes and lessons learned in the M. tuberculosis P450 enzyme family
- Source :
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1814:76-87
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- CYP144 from Mycobacterium tuberculosis was expressed and purified. CYP144 demonstrates heme thiolate coordination in its ferric form, but the cysteinate is protonated to thiol in both the carbon monoxide-bound and ligand-free ferrous forms (forming P420 in the former). Tight binding of various azole drugs was shown, with affinity for miconazole ( K d = 0.98 μM), clotrimazole (0.37 μM) and econazole (0.78 μM) being highest. These azoles are also the trio with the highest affinity for the essential CYP121 and for the cholesterol oxidase CYP125 (essential for host infection), and have high potency as anti-mycobacterial drugs. Construction of a Mtb gene knockout strain demonstrated that CYP144 is not essential for growth in vitro . However the deletion strain was more sensitive to azole inhibition in culture suggesting an important role for CYP144 in cell physiology and/or in mediating azole resistance. The biophysical and genetic features of CYP144 are compared to those of other characterized Mtb P450s, identifying both commonality in properties (including thiolate protonation in ferrous P450s) and intriguing differences in thermodynamic and spectroscopic features. Our developing knowledge of the Mtb P450s has revealed unusual biochemistry and gene essentiality, highlighting their potential as drug targets in this human pathogen.
- Subjects :
- Time Factors
Miconazole
Biophysics
Human pathogen
Biology
Spectrum Analysis, Raman
Binding, Competitive
Biochemistry
Analytical Chemistry
Mycobacterium tuberculosis
Gene Knockout Techniques
chemistry.chemical_compound
Bacterial Proteins
Cytochrome P-450 Enzyme System
Escherichia coli
Clotrimazole
Molecular Biology
Heme
Gene
Gene knockout
chemistry.chemical_classification
Cytochrome P450
biology.organism_classification
Recombinant Proteins
In vitro
Kinetics
chemistry
Spectrophotometry
Mutation
Anti-Infective Agents, Local
Potentiometry
biology.protein
Azole
Electrophoresis, Polyacrylamide Gel
Econazole
Oxidation-Reduction
Cell Division
Protein Binding
Subjects
Details
- ISSN :
- 15709639
- Volume :
- 1814
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- Accession number :
- edsair.doi.dedup.....e097cdfa3b323a822d64b7c7d645c683