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Characterization of the highly immunogenic VP2 protrusion domain as a diagnostic antigen for members of Birnaviridae family
- Source :
- Applied Microbiology and Biotechnology
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Birnaviridae is a family of viruses (birnaviruses) which consists of four genera, members of which cause diseases in fish, birds, mollusks, and insects. The genome of birnaviruses encodes the highly immunogenic VP2 capsid protein. In order to demonstrate that the VP2 protein can be exploited as a diagnostic antigen for birnaviruses, we developed a lateral flow assay based on the surface-exposed VP2 protrusion domain of a representative birnavirus, infectious bursal disease virus (IBDV) of serotype 1 which causes the highly devastating infectious bursal disease in chickens. The biophysical characterization of the purified domain reveals that the domain predominantly consists of β-sheets, exists in a trimeric form, and remains folded at high temperatures, making it suitable for diagnostic purposes. Owing to its highly immunogenic nature and excellent biophysical properties, we employed the VP2 protrusion domain in a gold nanoparticle-based lateral flow assay for rapid detection of anti-IBDV antibodies in serum samples of infected chickens. Our results indicate that the domain binds anti-IBDV antibodies with high specificity during laboratory testing and on-site testing. The lateral flow assay reported here yields comparable results in a qualitative manner as obtained through a commercial enzyme-linked immunosorbent assay (ELISA). As VP2 is a common capsid protein of birnaviruses, the lateral flow assay can be generalized for other birnaviruses, and members of Tetraviridae and Nodaviridae families which contain homologous VP2 capsid proteins. Electronic supplementary material The online version of this article (10.1007/s00253-020-10458-6) contains supplementary material, which is available to authorized users.
- Subjects :
- VP2 protrusion domain
animal structures
Birnaviridae
viruses
Metal Nanoparticles
Antibodies, Viral
Infectious bursal disease virus
Applied Microbiology and Biotechnology
Birnavirus
Virus
Infectious bursal disease
03 medical and health sciences
Protein Domains
Antigen
Spectroscopy, Fourier Transform Infrared
medicine
Animals
Biotechnologically Relevant Enzymes and Proteins
Gold nanoparticle-based lateral flow assay
Infrared spectroscopy
Antigens, Viral
Poultry Diseases
Tetraviridae
030304 developmental biology
Immunoassay
Viral Structural Proteins
0303 health sciences
biology
030306 microbiology
Circular Dichroism
General Medicine
Birnaviridae Infections
biology.organism_classification
medicine.disease
Virology
Capsid
biology.protein
Binding Sites, Antibody
Gold
Nodaviridae
Antibody
Chickens
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....e09542260dc81a812fb987ca50a70174