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Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm,Bombyx mori
- Source :
- Genome. 53:45-54
- Publication Year :
- 2010
- Publisher :
- Canadian Science Publishing, 2010.
-
Abstract
- We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori . In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1–21.5) and od (1–49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.
- Subjects :
- Genetic Markers
Male
Locus (genetics)
Chromosomal rearrangement
Biology
Models, Biological
Genetics
Animals
Cloning, Molecular
Molecular Biology
Sex Chromosome Aberrations
Sequence Deletion
Bombyx
Bacterial artificial chromosome
Z chromosome
Sex Chromosomes
Somatotypes
fungi
Chromosome Mapping
Chromosome
Chromosome Breakage
General Medicine
biology.organism_classification
Molecular biology
Eye pigmentation
Phenotype
Genetic Loci
Larva
Female
Chromosome breakage
Biotechnology
Subjects
Details
- ISSN :
- 14803321 and 08312796
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Genome
- Accession number :
- edsair.doi.dedup.....e08cbf06bfd1e82bfffee6795b76887a
- Full Text :
- https://doi.org/10.1139/g09-083