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Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm,Bombyx mori

Authors :
Kimiko Yamamoto
Toru Shimada
Susumu Katsuma
Akio Ohnuma
Kazuei Mita
Hiroaki Abe
Seigo Kuwazaki
Tsuguru FujiiT. Fujii
Source :
Genome. 53:45-54
Publication Year :
2010
Publisher :
Canadian Science Publishing, 2010.

Abstract

We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori . In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1–21.5) and od (1–49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.

Details

ISSN :
14803321 and 08312796
Volume :
53
Database :
OpenAIRE
Journal :
Genome
Accession number :
edsair.doi.dedup.....e08cbf06bfd1e82bfffee6795b76887a
Full Text :
https://doi.org/10.1139/g09-083