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Systemic disease in Peromyscus leucopus associated with Borrelia burgdorferi infection
- Source :
- The American journal of tropical medicine and hygiene. 42(3)
- Publication Year :
- 1990
-
Abstract
- Sixteen wild Peromyscus leucopus, trapped for the establishment of a breeding colony, developed signs of neurological damage (trembling, incoordination, circling, head tilt, and lameness of the rear legs) 2–47 days after capture in southern Wisconsin. Spirochetes were cultured from the brain of 5/11 mice, and Borrelia burgdorferi was cultured from 1 brain. A spirochete was isolated from the bladder of 1 mouse. The spirochete was identified by fluorescent antibody staining with the monoclonal antibody specific for B. burgdorferi, H5332. Serum antibodies to the spirochete were found in 14/15 mice. Negative results were obtained in all tests for viruses and bacteria, including Listeria (2/2), Mycoplasma (2/2), mouse hepatitis virus (10/10), Theilers's encephalomyelitis virus (GD VII) (8/8), REO 3 virus (2/2), and lymphocytic choriomeningitis virus (4/4). There was no bacterial growth from brains cultured on eosin methylene blue or blood agar (3/3). Histologic lesions included nonsuppurative cellular infiltrates in the brain, kidney, liver, and lung. Three outbred Swiss-Webster mice were inoculated orally with a suspension of the brain in BSKII medium, and 3 were inoculated with unpassed B. burgdorferi cultured from the brain of a P. leucopus with motor dysfunction. Five of the inoculated mice developed antibody titers of 1:128; one mouse was positive at 1:256. Motor signs of neurologic damage developed in 3/6 mice 2–24 weeks post-inoculation, and B. burgdorferi was detected in the brains of 2 mice by isolation and by fluorescent antibody.
- Subjects :
- Male
Peromyscus
Encephalomyelitis
Spirochaetaceae
Virus
Rodent Diseases
Mice
Mouse hepatitis virus
Borrelia burgdorferi Group
Virology
medicine
Animals
Borrelia burgdorferi
Lyme Disease
biology
Antibody titer
Borrelia Burgdorferi Infection
Brain
biology.organism_classification
medicine.disease
Antibodies, Bacterial
Infectious Diseases
Parasitology
Female
Nervous System Diseases
Subjects
Details
- ISSN :
- 00029637
- Volume :
- 42
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The American journal of tropical medicine and hygiene
- Accession number :
- edsair.doi.dedup.....e072f0f8c7019bfffa80a1fed35dcea3