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Ultradeep Bisulfite Sequencing Analysis of DNA Methylation Patterns in Multiple Gene Promoters by 454 Sequencing
- Source :
- Cancer Research. 67:8511-8518
- Publication Year :
- 2007
- Publisher :
- American Association for Cancer Research (AACR), 2007.
-
Abstract
- We developed a novel approach for conducting multisample, multigene, ultradeep bisulfite sequencing analysis of DNA methylation patterns in clinical samples. A massively parallel sequencing-by-synthesis method (454 sequencing) was used to directly sequence >100 bisulfite PCR products in a single sequencing run without subcloning. We showed the utility, robustness, and superiority of this approach by analyzing methylation in 25 gene-related CpG rich regions from >40 cases of primary cells, including normal peripheral blood lymphocytes, acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (CLL), follicular lymphoma (FL), and mantle cell lymphoma (MCL). A total of 294,631 sequences was generated with an average read length of 131 bp. On average, >1,600 individual sequences were generated for each PCR amplicon far beyond the few clones (
- Subjects :
- Cancer Research
Genome, Human
Lymphoma, Non-Hodgkin
Bisulfite sequencing
Sequence Analysis, DNA
Methylation
DNA Methylation
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Biology
Leukemia, Lymphocytic, Chronic, B-Cell
Polymorphism, Single Nucleotide
Molecular biology
Bisulfite
Oncology
DNA methylation
Humans
Sulfites
Illumina Methylation Assay
CpG Islands
Epigenetics
Methylated DNA immunoprecipitation
Promoter Regions, Genetic
Exome sequencing
Subjects
Details
- ISSN :
- 15387445 and 00085472
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Cancer Research
- Accession number :
- edsair.doi.dedup.....e06e902d126b5e3043e3cba602f9fe63