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A novel gene encoding a sulfur-regulated outer membrane protein in Thiobacillus ferrooxidans
- Publication Year :
- 1999
- Publisher :
- ELSEVIER SCIENCE BV, 1999.
-
Abstract
- Thiobacillus ferrooxidans is a Gram-negative chemolithotrophic bacterium able to oxidize ferrous iron, elemental sulfur and inorganic sulfur compounds. The oxidation of sulfur by T. ferrooxidans resulted in an expression of some outer membrane proteins (OMPs) at a level higher than that observed during ferrous iron oxidation. Among these OMPs, a protein with a molecular mass of 54 kDa was purified and 18 amino acids of the N-terminal sequence determined. Using a 54 bp PCR generated DNA product as a probe for the protein, we isolated a 4.5 kb Pst I DNA chromosomal fragment containing the corresponding gene. Sequencing 2169 bp of this fragment revealed the open reading frame codifying for the protein, consisting of 467 amino acids and a molecular mass of 49 674 Da. The mature protein was produced by the removal of a 32 amino acid signal peptide-like sequence from the N-terminus of a 499 amino acid peptide. Although no significant homology with any known protein has been found and its physiological role remains unclear, its high expression on sulfur substrates suggests a role in sulfide mineral oxidation.
- Subjects :
- DNA, Bacterial
Molecular Sequence Data
chemistry.chemical_element
Bioengineering
Biology
Applied Microbiology and Biotechnology
chemistry.chemical_compound
Bacterial Proteins
Amino Acid Sequence
Cloning, Molecular
Gene
chemistry.chemical_classification
Molecular mass
Base Sequence
Nucleic acid sequence
outer membrane proteins
sulfur oxidation
thiobacillus ferrooxidans
General Medicine
Gene Expression Regulation, Bacterial
Thiobacillus
Sulfur
Amino acid
Open reading frame
chemistry
Biochemistry
Bacterial outer membrane
DNA
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e05e93ab88c872a2c4419b51c6cf2332