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Selenoprotein Expression in Hürthle Cell Carcinomas and in the Human Hürthle Cell Carcinoma Line XTC.UC1
- Source :
- Thyroid. 15:405-416
- Publication Year :
- 2005
- Publisher :
- Mary Ann Liebert Inc, 2005.
-
Abstract
- Hürthle cell carcinomas (HTC) are characterized by mitochondrial amplification and enhanced oxygen metabolism. To clarify if defects in enzymes scavenging reactive oxygen species are involved in the pathogenesis of HTC, we analyzed selenium (Se)-dependent expression of various detoxifying selenoproteins in the HTC cell line XTC.UC1. Glutathione peroxidase and thioredoxin reductase activity was found both in cell lysates and conditioned media of XTC.UC1 cells and was increased by Na(2)SeO(3). Western blot analysis demonstrated the presence of thioredoxin reductase both in cell lysates and conditioned media and of glutathione peroxidase 3 in conditioned media. Type I 5'-deiodinase, another selenoprotein that catalyzes thyroid hormone metabolism, was detectable only in cell lysates by enzyme assay and Western blot, and responded to stimulation by both Na(2)SeO(3) and retinoic acid. A selenoprotein P signal was detected in conditioned media by Western blot, but was not enhanced by Na(2)SeO(3) treatment. In situ hybridization revealed glutathione peroxidase mRNAs in HTC specimen; glutathione peroxidase 3 mRNA levels were reduced. These data suggest adequate expression and Se-dependent regulation of a couple of selenoproteins involved in antioxidant defense and thyroid hormone metabolism in XTC.UC1 cells, so far giving no evidence of a role of these proteins in the pathogenesis of HTCs.
- Subjects :
- DNA, Complementary
Thioredoxin-Disulfide Reductase
Endocrinology, Diabetes and Metabolism
Blotting, Western
Cell
Biology
Iodide Peroxidase
Endocrinology
Cell Line, Tumor
Selenoprotein P
medicine
Adenoma, Oxyphilic
Humans
Thyroid Neoplasms
Selenoproteins
In Situ Hybridization
chemistry.chemical_classification
Glutathione Peroxidase
Reactive oxygen species
Oxygen metabolism
Proteins
Free Radical Scavengers
Molecular biology
digestive system diseases
Peroxides
medicine.anatomical_structure
Enzyme
chemistry
Culture Media, Conditioned
Hurthle cell carcinoma
Selenoprotein
Subjects
Details
- ISSN :
- 15579077 and 10507256
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Thyroid
- Accession number :
- edsair.doi.dedup.....e05e657c2c5360913d35fbbc69ec632f