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Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
- Publication Year :
- 2018
-
Abstract
- The brain’s extracellular matrix (ECM) is a macromolecular network composed of glycosaminoglycans, proteoglycans, glycoproteins, and fibrous proteins. In vitro studies often use purified ECM proteins for cell culture coatings, however these may not represent the molecular complexity and heterogeneity of the brain’s ECM. To address this, we compared neural network activity (over 30 days in vitro) from primary neurons co-cultured with glia grown on ECM coatings from decellularized brain tissue (bECM) or MaxGel, a non-tissue-specific ECM. Cells were grown on a multi-electrode array (MEA) to enable noninvasive long-term interrogation of neuronal networks. In general, the presence of ECM accelerated the formation of networks without affecting the inherent network properties. However, specific features of network activity were dependent on the type of ECM: bECM enhanced network activity over a greater region of the MEA whereas MaxGel increased network burst rate associated with robust synaptophysin expression. These differences in network activity were not attributable to cellular composition, glial proliferation, or astrocyte phenotypes, which remained constant across experimental conditions. Collectively, the addition of ECM to neuronal cultures represents a reliable method to accelerate the development of mature neuronal networks, providing a means to enhance throughput for routine evaluation of neurotoxins and novel therapeutics.
- Subjects :
- 0301 basic medicine
Patch-Clamp Techniques
Synaptophysin
lcsh:Medicine
Action Potentials
Article
Extracellular matrix
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Cellular neuroscience
medicine
Biological neural network
Animals
lcsh:Science
Electrodes
Cells, Cultured
Cell Proliferation
Automation, Laboratory
Neurons
Multidisciplinary
Decellularization
biology
Chemistry
lcsh:R
Brain
Hydrogels
Coculture Techniques
Cell biology
Extracellular Matrix
Rats
030104 developmental biology
medicine.anatomical_structure
Cell culture
biology.protein
lcsh:Q
Neuron
Nerve Net
Neuroglia
030217 neurology & neurosurgery
Astrocyte
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....e05bdeedc9272362d57a744492e0776b