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Adipocyte-specific deletion of IL-6 does not attenuate obesity-induced weight gain or glucose intolerance in mice
- Source :
- American Journal of Physiology-Endocrinology and Metabolism. 317:E597-E604
- Publication Year :
- 2019
- Publisher :
- American Physiological Society, 2019.
-
Abstract
- It has been suggested that interleukin-6 (IL-6) produced by adipocytes in obesity leads to liver insulin resistance, although this hypothesis has never been definitively tested. Accordingly, we did so by generating adipocyte-specific IL-6-deficient (AdipoIL-6−/−) mice and studying them in the context of diet-induced and genetic obesity. Mice carrying two floxed alleles of IL-6 (C57Bl/6J) were crossed with Cre recombinase-overexpressing mice driven by the adiponectin promoter to generate AdipoIL-6−/− mice. AdipoIL-6−/− and floxed littermate controls were fed a standard chow or high-fat diet (HFD) for 16 wk and comprehensively metabolically phenotyped. In addition to a diet-induced obesity model, we also examined the role of adipocyte-derived IL-6 in a genetic model of obesity and insulin resistance by crossing the AdipoIL-6−/− mice with leptin-deficient ( ob/ob) mice. As expected, mice on HFD and ob/ob mice displayed marked weight gain and increased fat mass compared with chow-fed and ob/+ (littermate control) animals, respectively. However, deletion of IL-6 from adipocytes in either model had no effect on glucose tolerance or fasting hyperinsulinemia. We concluded that adipocyte-specific IL-6 does not contribute to whole body glucose intolerance in obese mice.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Physiology
Fasting hyperinsulinemia
Endocrinology, Diabetes and Metabolism
Context (language use)
Biology
Diet, High-Fat
Weight Gain
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Insulin resistance
Non-alcoholic Fatty Liver Disease
Physiology (medical)
Internal medicine
Adipocyte
Diabetes mellitus
Glucose Intolerance
Genetic model
Adipocytes
medicine
Animals
Obesity
Adiposity
Mice, Knockout
Adiponectin
Interleukin-6
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
chemistry
Body Composition
Insulin Resistance
medicine.symptom
Weight gain
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15221555 and 01931849
- Volume :
- 317
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Endocrinology and Metabolism
- Accession number :
- edsair.doi.dedup.....e037cc79a28725cd9841afdb5be25776