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Coupling of adrenal medullary opioid receptors to islet-activating protein-sensitive GTP-binding proteins
- Source :
- Life sciences. 40(18)
- Publication Year :
- 1987
-
Abstract
- Possible coupling of bovine adrenal medullary opioid receptors to islet-activating protein (IAP, pertussis toxin)-sensitive GTP-binding proteins was investigated by studying effects of guanyl-5'-yl imidodiphosphate (Gpp(NH)p) and IAP treatment of membranes on opioid binding. Gpp(NH)p inhibited [3H]D-Ala2-D-Leu5-enkephalin ([3H]DADLE) binding by increasing the dissociation constant of [3H]DADLE and membranes, and enhanced slightly [3H]diprenorphine binding. IAP treatment of membranes reduced [3H]DADLE binding and abolished almost completely the Gpp(NH)p inhibition of [3H]DADLE binding. Treatment of membranes with IAP and [32P]NAD resulted in radio-labeling of membrane proteins of approximately 39,000 dalton. DADLE inhibited adenylate cyclase activity in rat brain caudate nucleus. However, DADLE, beta-endorphin, levorphanol and dynorphin A(1-13) did not show any significant inhibitory action on bovine adrenal medullary adenylate cyclase activity. These results suggest that bovine adrenal medullary opioid (DADLE) receptors are linked to IAP-sensitive GTP-binding proteins which are not directly coupled to adenylate cyclase.
- Subjects :
- medicine.medical_specialty
Diprenorphine
Cyclase
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
GTP-Binding Proteins
Internal medicine
medicine
Animals
Virulence Factors, Bordetella
General Pharmacology, Toxicology and Pharmaceutics
Opioid peptide
Guanylyl Imidodiphosphate
Chemistry
Dynorphin A
General Medicine
Enkephalin, Leucine-2-Alanine
medicine.anatomical_structure
Endocrinology
Pertussis Toxin
Adrenal Medulla
Adenylate Cyclase Toxin
Adenylyl Cyclase Inhibitors
Receptors, Opioid
DADLE
Cattle
Adrenal medulla
Cyclase activity
medicine.drug
Enkephalin, Leucine
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 40
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....e023c2fb276d796b4bb396b9121c4bfb