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Epidermal growth factor alters the bax:bcl-w ratio following massive small bowel resection
- Source :
- The Journal of surgical research. 91(1)
- Publication Year :
- 2000
-
Abstract
- Background. Following massive small bowel resection (SBR), the expression of bax and bcl-w is associated with increased enterocyte apoptosis. Epidermal growth factor (EGF) has been shown to enhance enterocyte proliferation and retard apoptosis in the adapting bowel. This study examined the effect of EGF on the expression of these bcl-2 family members during adaptation. Materials and methods. Mice (C57Bl/6; n = 38) underwent a 50% SBR or sham operation and then were randomized to receive twice-daily orogastric saline or EGF (50 μg/kg/day). After 3 days, the remnant ileum was removed, apoptotic index (No. apoptotic bodies/crypt) calculated, and expression of mRNA and protein for bax and bcl-w quantified. Results. EGF prevented the expected increase in the apoptotic index after SBR and altered the ratio of bax to bcl-w in favor of cell survival. Conclusion. Following massive small bowel resection, EGF retards rates of enterocyte apoptosis and modifies the expression of bcl-2 family members. By decreasing bax and increasing bcl-w expression, the balance between pro- and anti-apoptotic genes is shifted in favor of cell survival. Alteration of bcl-2 family member expression may be an important mechanism by which EGF reduces the increased enterocyte apoptosis that occurs after massive small bowel resection.
- Subjects :
- Male
Short Bowel Syndrome
medicine.medical_specialty
Enterocyte
Cell Survival
medicine.medical_treatment
Crypt
Blotting, Western
Gene Expression
Ileum
Apoptosis
Biology
Andrology
Mice
Epidermal growth factor
Internal medicine
Proto-Oncogene Proteins
Intestine, Small
medicine
Animals
RNA, Messenger
bcl-2-Associated X Protein
Epidermal Growth Factor
Growth factor
Adaptation, Physiological
Small intestine
Mice, Inbred C57BL
medicine.anatomical_structure
Cytokine
Endocrinology
Enterocytes
Proto-Oncogene Proteins c-bcl-2
Surgery
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 91
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of surgical research
- Accession number :
- edsair.doi.dedup.....e0235e7233f740e693ed29af5410c339