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5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

Authors :
Hana Šanderová
Libor Krásný
Michal Hocek
Dragana Vítovská
Martina Janoušková
Fabrizia Nici
Zuzana Vaníková
Soňa Boháčová
Source :
Chemical communications (Cambridge, England). 53(99)
Publication Year :
2017

Abstract

DNA templates containing 5-hydroxymethyluracil or 5-hydroxymethylcytosine were used in an in vitro transcription assay with RNA polymerase from Escherichia coli. A strong enhancement of transcription was observed from DNA containing the Pveg promoter whereas a decrease was observed from DNA containing the rrnB P1 promoter, suggesting that they may act as epigenetic marks.

Details

ISSN :
1364548X
Volume :
53
Issue :
99
Database :
OpenAIRE
Journal :
Chemical communications (Cambridge, England)
Accession number :
edsair.doi.dedup.....e017a82ebbd84168000851a5e01d4fe2