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Electrostatic Interaction on Loading of Therapeutic Peptide GLP-1 into Porous Silicon Nanoparticles
- Source :
- Langmuir. 31:1722-1729
- Publication Year :
- 2015
- Publisher :
- American Chemical Society (ACS), 2015.
-
Abstract
- Porous silicon (PSi) nanoparticles' tunable properties are facilitating their use at highly challenging medical tasks such as peptide delivery. Because of many different mechanisms that are affecting the interaction between the peptide and the particle, the drug incorporation into the mesoporous delivery system is not straightforward. We have studied the adsorption and loading of incretin hormone glucagon like peptide 1 (GLP-1) on PSi nanoparticles. The results show that the highest loading degree can be achieved in pH values near the isoelectric point of peptide, and the phenomenon is independent of the surface's zeta potential. In order to study the interaction between the peptide and the nanoparticle, we studied the adsorption with lower concentrations and noticed that also non-Coulombic forces have a big role in adsorption of GLP-1. Adsorption is effective and pH-independent especially on low peptide concentrations and onto more hydrophobic nanoparticles. Reversibility of adsorption was studied as a function of buffer pH. When the loading is compared to the total mass of the formulation, the loading degree is 29%, and during desorption experiments 25% is released in 4 h and can be considered as a reversible loading degree. Thus, the peptides adsorbed first seem to create irreversibly adsorbed layer that facilitates reversible adsorption of following peptides.
- Subjects :
- Silicon
Surface Properties
Molecular Sequence Data
Nanoparticle
Peptide
Porous silicon
Adsorption
Glucagon-Like Peptide 1
Electrochemistry
Zeta potential
General Materials Science
Amino Acid Sequence
Spectroscopy
chemistry.chemical_classification
Chromatography
ta114
Chemistry
Surfaces and Interfaces
Hydrogen-Ion Concentration
Condensed Matter Physics
Isoelectric point
Chemical engineering
Nanoparticles
Particle
Mesoporous material
Porosity
Subjects
Details
- ISSN :
- 15205827 and 07437463
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Langmuir
- Accession number :
- edsair.doi.dedup.....e0174adac8447baebd5cdc647caffecc