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Platelet-rich plasma improves therapeutic effects of menstrual blood-derived stromal cells in rat model of intrauterine adhesion
- Source :
- Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-12 (2019), Stem Cell Research & Therapy
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Background Intrauterine adhesion (IUA) is a major cause of female secondary infertility. We previously demonstrated that menstrual blood-derived stromal cell (MenSC) transplantation helped severe IUA patients have pregnancy and endometrium regeneration. We also initiated platelet-rich plasma (PRP) acted as a beneficial supplement in MenSC culturing and a potential endometrial receptivity regulator. Here, we investigated the therapeutic effect of combined transplantation of MenSCs with PRP in rat IUA models and the mechanisms of MenSCs in endometrium regeneration. Methods Rat IUA models were established by intrauterine mechanical injured. Nine days later, all rats were randomly assigned to four groups received different treatment: placebo, MenSC transplantation, PRP transplantation, and MenSCs + PRP transplantation. The traces of MenSCs were tracked with GFP label. Endometrial morphology and pathology, tissue proliferation, inflammation, pregnancy outcomes, and mechanism of MenSCs in the regeneration of endometrium were investigated. Results Notably, at days 9 and 18 post-treatment, MenSC transplantation significantly improved endometrial proliferation, angiogenesis, and morphology recovery and decreased collagen fibrosis and inflammation in the uterus. MenSCs had lesion chemotaxis, colonized around the endometrial glands. Gene expression of human-derived secretory protein IGF-1, SDF-1, and TSP-1 was detected in the uterus received MenSCs at day 18. The three treatments can all improve fertility in IUA rats. Moreover, gene expressions of cell proliferation, developmental processes, and other biological processes were induced in MenSC transplantation group. Hippo signaling pathway was the most significantly changed pathway, and the downstream factors CTGF, Wnt5a, and Gdf5 were significantly regulated in treatment groups. PRP enhanced these parameters through a synergistic effect. Conclusions In summary, MenSCs could effectively improve uterine proliferation, markedly accelerate endometrial damage repairment and promote fertility restoration in IUA rats, suggesting a paracrine restorative effect and Hippo signaling pathway stimulation. Our results indicate MenSCs, a valuable source of cells for transplantation in the treatment intrauterine adhesion. Combined with PRP, this cell therapy was more effective. Electronic supplementary material The online version of this article (10.1186/s13287-019-1155-7) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
PRP
Angiogenesis
Uterus
Medicine (miscellaneous)
Endometrium
Thrombospondin 1
0302 clinical medicine
Pregnancy
lcsh:QD415-436
Insulin-Like Growth Factor I
Intrauterine adhesion
Uterine Diseases
lcsh:R5-920
Platelet-Rich Plasma
Pregnancy Outcome
Gene Expression Regulation, Developmental
Menstruation
medicine.anatomical_structure
030220 oncology & carcinogenesis
Rat model
Molecular Medicine
Female
lcsh:Medicine (General)
Signal Transduction
Stromal cell
MenSCs
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Andrology
lcsh:Biochemistry
03 medical and health sciences
Paracrine signalling
Cell Adhesion
medicine
Animals
Humans
Regeneration
Cell Proliferation
Inflammation
business.industry
Research
Cell Biology
Chemokine CXCL12
Rats
CTGF
Transplantation
030104 developmental biology
Platelet-rich plasma
Stromal Cells
business
Subjects
Details
- Language :
- English
- ISSN :
- 17576512
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....e01700dbac9f5ed1f17693ea7648fe31