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Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling
- Source :
- UPCommons. Portal del coneixement obert de la UPC, Universitat Politècnica de Catalunya (UPC), Recercat. Dipósit de la Recerca de Catalunya, instname
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M(3), M(4), and M(5) subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M(3) subtype in a G(q/11)-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M(3) and M(5) both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.
- Subjects :
- Cellular signal transduction
Ciències de la salut::Medicina [Àrees temàtiques de la UPC]
T-Lymphocytes
T cell
Immunology
Muscarinic receptors
Muscarinic Antagonists
Biology
Jurkat cells
Limfòcits
Jurkat Cells
Receptors
Muscarinic acetylcholine receptor
medicine
Humans
Immunology and Allergy
Lymphocytes
Immunologia
Receptor
Acetylcholine receptor
Receptor, Muscarinic M3
Receptor, Muscarinic M2
Receptor, Muscarinic M5
Receptor, Muscarinic M4
Immunopharmacology
Receptor, Muscarinic M1
Muscarinic acetylcholine receptor M3
Receptors, Muscarinic
Cell biology
Ciències de la salut::Medicina::Farmacologia [Àrees temàtiques de la UPC]
medicine.anatomical_structure
Neurology
Second messenger system
G proteins--Receptors
Proteïnes G -- Receptors
Neurology (clinical)
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 237
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....dfe3e9a38b4deee082d51e3cc95c5673