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Resistance gene analogues identified through the NBS-profiling method map close to major genes and QTL for disease resistance in apple
- Source :
- HAL, TAG Theoretical and Applied Genetics, TAG Theoretical and Applied Genetics, Springer Verlag, 2005, 110, pp.660-668, Theoretical and Applied Genetics, 110(4), 660-668, Theoretical and Applied Genetics 110 (2005) 4
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Abstract
- We used a new method called nucleotide-binding site (NBS) profiling to identify and map resistance gene analogues (RGAs) in apple. This method simultaneously allows the amplification and the mapping of genetic markers anchored in the conserved NBS-encoding domain of plant disease resistance genes. Ninety-four individuals belonging to an F1 progeny derived from a cross between the apple cultivars ‘Discovery’ and ‘TN10-8’ were studied. Two degenerate primers designed from the highly conserved P-loop motif within the NBS domain were used together with adapter primers. Forty-three markers generated with NBS profiling could be mapped in this progeny. After sequencing, 23 markers were identified as RGAs, based on their homologies with known resistance genes or NBS/leucine-rich-repeat-like genes. Markers were mapped on 10 of the 17 linkage groups of the apple genetic map used. Most of these markers were organized in clusters. Twenty-five markers mapped close to major genes or quantitative trait loci for resistance to scab and mildew previously identified in different apple progenies. Several markers could become efficient tools for marker-assisted selection once converted into breeder-friendly markers. This study demonstrates the efficiency of the NBS-profiling method for generating RGA markers for resistance loci in apple.
- Subjects :
- 0106 biological sciences
Genetic Markers
defense genes
scab resistance
Molecular Sequence Data
Quantitative Trait Loci
[SDV.GEN] Life Sciences [q-bio]/Genetics
Quantitative trait locus
Plant disease resistance
progeny
Genes, Plant
01 natural sciences
Chromosomes, Plant
03 medical and health sciences
PRI Biodiversiteit en Veredeling
Arabidopsis
Genetics
mildew resistance
Amino Acid Sequence
Gene
030304 developmental biology
Plant Diseases
2. Zero hunger
0303 health sciences
[SDV.GEN]Life Sciences [q-bio]/Genetics
biology
venturia-inaequalis
Venturia inaequalis
food and beverages
Chromosome Mapping
General Medicine
biology.organism_classification
Major gene
homologs
PRI Bioscience
PRI Biodiversity and Breeding
arabidopsis
co-localize
Genetic marker
Malus
quantitative trait loci
potato
Agronomy and Crop Science
Powdery mildew
Genome, Plant
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 00405752 and 14322242
- Database :
- OpenAIRE
- Journal :
- HAL, TAG Theoretical and Applied Genetics, TAG Theoretical and Applied Genetics, Springer Verlag, 2005, 110, pp.660-668, Theoretical and Applied Genetics, 110(4), 660-668, Theoretical and Applied Genetics 110 (2005) 4
- Accession number :
- edsair.doi.dedup.....dfaa7b83cbefe75b33e3772b70a20b02