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Long-term tobacco exposure and immunosenescence: Paradoxical effects on T-cells telomere length and telomerase activity
- Source :
- Mechanisms of Ageing and Development. 197:111501
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Immunosenescence are alterations on immune system that occurs throughout an individual life. The main characteristic of this process is replicative senescence, evaluated by telomere shortening. Several factors implicate on telomere shortening, such as smoking. In this study, we evaluated the influence of smoking and Chronic Obstructive Pulmonary Disease (COPD) on cytokines, telomere length and telomerase activity. Blood samples were collected from subjects aged over 60 years old: Healthy (never smokers), Smokers (smoking for over 30 years) and COPDs (ex-smokers for ≥15 years). A young group was included as control. PBMCs were cultured for assessment of telomerase activity using RT-PCR, and cytokines secretion flow cytometry. CD4+ and CD8+ purified lymphocytes were used to assess telomere length using FlowFISH. We observed that COPD patients have accelerated telomere shortening. Paradoxically, smokers without lung damage showed preserved telomere length, suggesting that tobacco smoking may affect regulatory mechanisms, such as telomerase. Telomerase activity showed diminished activity in COPDs, while Smokers showed increased activity compared to COPDs and Healthy groups. Extracellular environment reflected this unbalance, indicated by an anti-inflammatory profile in Smokers, while COPDs showed an inflammatory prone profile. Further studies focusing on telomeric maintenance may unveil mechanisms that are associated with cancer under long-term smoking.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
0301 basic medicine
Senescence
Aging
Telomerase
Immunosenescence
CD8-Positive T-Lymphocytes
Pulmonary Disease, Chronic Obstructive
03 medical and health sciences
0302 clinical medicine
Immune system
medicine
Humans
Longitudinal Studies
Telomere Shortening
Aged
COPD
business.industry
Smoking
Cancer
Middle Aged
medicine.disease
respiratory tract diseases
Telomere
030104 developmental biology
Immunology
Female
business
030217 neurology & neurosurgery
CD8
Developmental Biology
Subjects
Details
- ISSN :
- 00476374
- Volume :
- 197
- Database :
- OpenAIRE
- Journal :
- Mechanisms of Ageing and Development
- Accession number :
- edsair.doi.dedup.....dfa901250efe33c5202ae0a261b88f19