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Identification of the hallmarks of necroptosis and ferroptosis by transmission electron microscopy
- Source :
- Biochemical and Biophysical Research Communications. 527:839-844
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Apoptosis is the prototype for a regulated form of cell death, but recent studies have revealed other types of regulated forms of cell death, including necroptosis and ferroptosis. The molecular mechanisms underlying the execution of these processes have been intensively investigated, yet the hallmarks of their morphology are not fully understood. Here, we report that electron lucent cytoplasm was a common feature of both necroptosis and ferroptosis, which was consistent with cytoplasmic vacuolization due to a defect in the cytoplasmic membrane integrity. Notably, the perinuclear space was dilated in necroptosis, but such dilation did not occur in ferroptosis. Cells undergoing ferroptosis, but not necroptosis, exhibited an electron lucent nucleus. We previously reported that one of the nuclear danger-associated molecular patterns (DAMPs), high mobility group box (HMGB)1, is rapidly released from the nucleus to the extracellular spaces of cells undergoing necroptosis through the ruptured nuclear and cytoplasmic membrane. Via time-lapse imaging of cells stably expressing HMGB1 fused to a fluorescence protein, we found that HMGB1 was also released from the nucleus to the cytosol, and then eventually released into the extracellular spaces in cells undergoing ferroptosis. Thus, nuclear membrane damage was induced prior to cytoplasmic membrane rupture in ferroptosis. Thus, dilation of the perinuclear space and an electron lucent nucleus may be the hallmarks of necroptosis and ferroptosis, respectively.
- Subjects :
- 0301 basic medicine
Cytoplasm
Programmed cell death
Necroptosis
Biophysics
HMGB1
Biochemistry
03 medical and health sciences
0302 clinical medicine
Microscopy, Electron, Transmission
Cell Line, Tumor
Extracellular
medicine
Ferroptosis
Humans
HMGB1 Protein
Nuclear membrane
Molecular Biology
Cell Nucleus
biology
Chemistry
Cell Membrane
Cell Biology
Cell biology
Cytosol
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
biology.protein
Nucleus
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 527
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....df9bbf092c54fc33cf11b695fa0f89dd