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Paper-Based Competitive Immunochromatography Coupled with an Enzyme-Modified Electrode to Enable the Wireless Monitoring and Electrochemical Sensing of Cotinine in Urine
- Source :
- Sensors, Vol 21, Iss 1659, p 1659 (2021), Sensors (Basel, Switzerland), Sensors, Volume 21, Issue 5
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- This paper proposes a combined strategy of using paper-based competitive immunochromatography and a near field communication (NFC) tag for wireless cotinine determination. The glucose oxidase labeled cotinine antibody specifically binds free cotinine in a sample, whereas the unoccupied antibody attached to BSA-cotinine at the test line on a lateral flow strip. The glucose oxidase on the strip and an assistant pad in the presence of glucose generated H2O2 and imposed the Ag oxidation on the modified electrode. This enabled monitoring of immunoreaction by either electrochemical measurement or wireless detection. Wireless sensing was realized for cotinine in the range of 100–1000 ng/mL (R2 = 0.96) in PBS medium. Undiluted urine samples from non-smokers exhibited an Ag-oxidation rate three times higher than the smoker’s urine samples. For 1:8 diluted urine samples (smokers), the proposed paper-based competitive immunochromatography coupled with an enzyme-modified electrode differentiated positive and negative samples and exhibited cotinine discrimination at levels higher than 12 ng/mL. This novel sensing platform can potentially be combined with a smartphone as a reader unit.
- Subjects :
- 02 engineering and technology
Urine
Biosensing Techniques
Electrochemistry
lcsh:Chemical technology
01 natural sciences
Biochemistry
Diluted urine
Article
Chromatography, Affinity
Analytical Chemistry
chemistry.chemical_compound
wireless biosensor
immunochromatography
Analytisk kemi
Glucose oxidase
lcsh:TP1-1185
cotinine
Electrical and Electronic Engineering
Instrumentation
Electrodes
nanomaterials
Chromatography
biology
010401 analytical chemistry
Paper based
Hydrogen Peroxide
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Test line
chemistry
Electrode
biology.protein
0210 nano-technology
Cotinine
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 21
- Issue :
- 1659
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....df96e04c3e5aef079c43a637b8fe19a6