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Distribution of Brain-Derived Neurotrophic Factor (BDNF) Protein and mRNA in the Normal Adult Rat CNS: Evidence for Anterograde Axonal Transport
- Source :
- The Journal of Neuroscience. 17:2295-2313
- Publication Year :
- 1997
- Publisher :
- Society for Neuroscience, 1997.
-
Abstract
- A sensitive immunohistochemical technique was used, along with highly specific affinity-purified antibodies to brain-derived neurotrophic factor (BDNF), to generate a detailed mapping of BDNF immunoreactivity (BDNF-ir) throughout the adult rat CNS. A parallel analysis of sites of BDNF synthesis was performed within situhybridization techniques using a cRNA probe to the exon encoding mature rat BDNF protein. These combined data revealed (1) groups of cell bodies containing diffuse BDNF-ir throughout the CNS that were strongly correlated with fields of cells containing BDNF mRNA; (2) varying degrees of BDNF-ir outside of cell bodies, in what appeared to be fibers and/or terminals; and (3) many regions containing extremely heavy BDNF-immunoreactive fiber/terminal labeling that lacked BDNF mRNA (e.g., medial habenula, central nucleus of the amygdala, bed nucleus of stria terminalis, lateral septum, and spinal cord). The latter observation suggested that in these regions BDNF was derived from anterograde axonal transport by afferent systems. In the two cases in which this hypothesis was tested by the elimination of select afferents, BDNF immunostaining was completely eliminated. These data, along with the observation that BDNF-ir was rarely found within dendrites or fibersen passage, suggest that BDNF protein produced in adult CNS neurons is polarized primarily along axonal processes and is preferentially stored in terminals within the innervation target.
- Subjects :
- Male
DNA, Complementary
In situ hybridization
Biology
Axonal Transport
Rats, Sprague-Dawley
Nerve Fibers
Reference Values
Neurotrophic factors
Animals
RNA, Messenger
In Situ Hybridization
Neurons
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor
General Neuroscience
Central nucleus of the amygdala
Brain
Articles
Immunohistochemistry
Anterograde axonal transport
Rats
Cell biology
Stria terminalis
Spinal Cord
nervous system
Organ Specificity
Axoplasmic transport
Female
Neuroscience
Immunostaining
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....df94139d5c729368f46b23e844341dc9