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Differential Gene Expression Analysis in European Eels (Anguilla anguilla, L. 1758) Naturally Infected by Macroparasites
- Source :
- Journal of Parasitology, Journal of Parasitology, American Society of Parasitologists, 2008, 94 (3), pp.571-577
- Publication Year :
- 2008
- Publisher :
- American Society of Parasitologists, 2008.
-
Abstract
- We analyzed the relationships between the macroparasite community of the European eel and the expression of genes involved in the host physiology during its continental life. The genes studied are implicated in (1) host response to environmental stress, i.e., heat shock protein 70 (HSP70) and metallothionein (MT); (2) osmoregulation, i.e., beta thyroid hormone receptor (betaTHR) and Na+/K+ATPase; and (3) silvering, i.e., betaTHR, freshwater rod opsin (FWO), and deep-sea rod opsin (DSO). All were enumerated by quantitative reverse-transcription polymerase chain reaction. The epizootiological results for 93 yellow eels caught in the Salses-Leucate Lagoon (France) included 11 species: 1 nematode, 2 acanthocephalans, 1 monogenean, and 7 digeneans. The molecular results revealed (1) a significant negative relationship between digenean abundance and the expression level of all the tested genes, except FWO; (2) a significant negative relationship between the abundance of the nematode Anguillicola crassus and the expression level of the Na+/K+ATPase gene; and (3) a significant positive relationship between the A. crassus abundance and the expression level of the MT gene. Eels infected with digeneans had, on average, a lower level of expressed genes. We hypothesize that the parasites may disturb the eel's ability to withstand environmental stress and delay their migration to the Sargasso Sea because of degeneration of the gut. We further propose that the effect of the invasive species, A. crassus, on the gene expression was mainly linked to an increased trophic activity of infected eels. Moreover, it is possible that the parasite may have an effect on the fish's migratory behavior, which is tied to reproductive purposes. Additional work, including an experimental approach, is required to confirm our hypotheses.
- Subjects :
- Gills
Parasitic Diseases, Animal
Gene Expression
Zoology
Parasitism
Biology
Eye
Fish Diseases
03 medical and health sciences
Stress, Physiological
Anguillidae
Gene expression
Prevalence
Animals
Parasite hosting
HSP70 Heat-Shock Proteins
14. Life underwater
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Ecology
Gene Expression Profiling
Rod Opsins
Thyroid Hormone Receptors beta
Aquatic animal
Silvering
04 agricultural and veterinary sciences
Water-Electrolyte Balance
Anguilla
biology.organism_classification
Nematode
Liver
040102 fisheries
0401 agriculture, forestry, and fisheries
Macroparasite
Metallothionein
Parasitology
Sodium-Potassium-Exchanging ATPase
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Subjects
Details
- ISSN :
- 19372345 and 00223395
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Journal of Parasitology
- Accession number :
- edsair.doi.dedup.....df76efdc22e55f4a72521c2937015fc1