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A pilot study of alterations in oxidized angiotensinogen and antioxidants in pre-eclamptic pregnancy
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- The oxidation status of angiotensinogen (AGT) may have a critical role in pre-eclampsia. We used a validated, quantitative, mass spectrometry-based method to measure the oxidized and total AGT levels in plasma of pre-eclamptic women (n = 17), normotensive-matched controls (n = 17), and healthy non-pregnant women (n = 10). Measurements of plasma glutathione peroxidase (GPx) activity and serum selenium concentrations were performed as markers of circulating antioxidant capacity. Higher proportions of oxidized AGT in plasma from pre-eclamptic women compared to matched normotensive pregnant controls (P = 0.006), whilst maintaining a similar total plasma AGT concentration were found. In the pre-eclamptic group, blood pressure were correlated with the proportion of oxidized AGT; no such correlation was seen in the normotensive pregnant women. Plasma GPx was inversely correlated with oxidized AGT, and there was an inverse association between serum selenium concentration and the proportion of oxidized AGT. This is the first time that oxidized AGT in human plasma has been linked directly to antioxidant status, providing a mechanism for the enhanced oxidative stress in pre-eclampsia. We now provide pathophysiological evidence that the conversion of the reduced form of AGT to its more active oxidized form is associated with inadequate antioxidant status and could indeed contribute to the hypertension of pre-eclampsia.
- Subjects :
- Proteomics
0301 basic medicine
Antioxidant
GPX3
Placenta
medicine.medical_treatment
Angiotensinogen
lcsh:Medicine
Blood Pressure
Pilot Projects
030204 cardiovascular system & hematology
medicine.disease_cause
Biochemistry
Antioxidants
0302 clinical medicine
Pre-Eclampsia
Pregnancy
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
Total plasma
Chemistry
Glutathione peroxidase
Pathophysiology
Enzymes
Hypertension
Female
Oxidoreductases
Oxidation-Reduction
Adult
medicine.medical_specialty
Article
Selenium
03 medical and health sciences
Medical research
Internal medicine
parasitic diseases
medicine
Humans
Glutathione Peroxidase
lcsh:R
Diagnostic markers
medicine.disease
Oxidative Stress
030104 developmental biology
Blood pressure
Endocrinology
Preclinical research
lcsh:Q
Biomarkers
Oxidative stress
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....df64346b2461abdacda06797b0144cc0