Back to Search
Start Over
Determination of cerebrospinal fluid leakage by selective deletion of transferrin glycoform using an immunochromatographic assay
- Source :
- Theranostics
- Publication Year :
- 2019
- Publisher :
- Ivyspring International Publisher, 2019.
-
Abstract
- Cerebrospinal fluid (CSF) leakage can lead to brain and spine pathologies and there is an urgent need for a rapid diagnostic method for determining CSF leakage. Beta-2 transferrin (β2TF), asialotransferrin, is a specific CSF glycoprotein biomarker used to determine CSF leakage when distinguished from serum sialotransferrin (sTF). Methods: We detected β2TF using an immunochromatographic assay (ICA), which can be potentially developed as a point-of-care (POC) testing platform. Sialic acid-specific lectin selectively captures sTF in multiple deletion lines within an ICA test strip, enabling the detection of β2TF. A sample pre-treatment process efficiently captures excess sTF increasing sensitivity for CSF leakage detection. Results: An optimal cut-off value for determining the presence of CSF in test samples was obtained from receiver operating characteristic (ROC) analysis of the ratio of the test signal intensity and the deletion lines. On 47 clinical samples, ICA test strips discriminated CSF positive from negative samples with statistically significant (positive versus negative t-test; P =0.00027). Additional artificial positive samples, prepared by mixing CSF positive and negative clinical samples, were used as a further challenge. These positive samples were clearly discriminated from the negative samples (mixture versus negative t-test; P =0.00103) and CSF leakage was determined with 97.1% specificity and 96.2% sensitivity. Conclusions: ICA represents a promising approach for POC diagnosis of CSF leakage. While requiring 70 min assay time inconvenient for POC testing, our method was significantly shorter than conventional electrophoresis-based detection methods for β2TF.
- Subjects :
- Diagnostic methods
Point-of-care testing
Medicine (miscellaneous)
Metal Nanoparticles
01 natural sciences
Test strips
chemistry.chemical_compound
0404 agricultural biotechnology
Cerebrospinal fluid
Humans
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Cerebrospinal Fluid Leakage
chemistry.chemical_classification
Immunoassay
point of care testing
Receiver operating characteristic
Cerebrospinal Fluid Leak
010401 analytical chemistry
Transferrin
04 agricultural and veterinary sciences
040401 food science
Molecular biology
N-Acetylneuraminic Acid
0104 chemical sciences
Sialic acid
chemistry
sialic acid
Point-of-Care Testing
cerebrospinal fluid leakage
Gold
immunochromatographic assay
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 18387640
- Volume :
- 9
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Theranostics
- Accession number :
- edsair.doi.dedup.....df4f251736b23717dc11b02ff84ade8a