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Interactions of BRCA1-mutated Breast Cancer Cell Lines with Adipose-derived Stromal Cells (ADSCs)
- Source :
- Journal of Mammary Gland Biology and Neoplasia
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- Lipofilling may constitute a technique to assist reconstruction of breasts following prophylactic mastectomy for patients with mutated BRCA1 or BRCA2 genes. However, to date it is not clear whether adipose-derived stromal cells (ADSCs) increase the risk of tumor initiation and progression in this situation. Therefore, the interactions of BRCA1 mutated breast cancer cell lines with normal ADSCs were investigated in the present study. Characteristics of MDA-MB-436 (BRCA1 c.5277 + 1G > A) and HCC1937 (BRCA1 p.Gln1756.Profs*74) were compared to MDA-MB-231 and T47D BRCA1/2 wild-type breast cancer cell lines. ADSCs were cultivated from lipoaspirates of a panel of BRCA1/2- wildtype patients. Interactions of conditioned medium (CM) of these cells with the breast cancer lines were studied using proliferation and migration assays as well as adipokine expression western blot arrays. CM of ADSCs exhibit a dose-dependent stimulation of the proliferation of the breast cancer cell lines. However, of the ADSC preparations tested, only 1 out of 18 samples showed a significant higher stimulation of BRCA1-mutated MDA-MB-436 versus wildtype MDA-MB-231 cells, and all CM revealed lower stimulatory activity for BRCA1-mutated HCC1937 versus wildtype T47D cells. Additionally, migration of breast cancer cells in response to CM of ADSCs proved to be equivalent or slower for BRCA1/2 mutated versus nonmutated cancer cells and, with exception of angiopoietin-like 2, induced expression of adipokines showed no major difference. Effects of media conditioned by normal ADSCs showed largely comparable effects on BRCA1-mutated and wildtype breast cancer cell lines thus advocating lipofilling, preferentially employing allogeneic non-mutated ADSCs.
- Subjects :
- 0301 basic medicine
Cancer Research
Stromal cell
Proliferation
Adipokine
Adipose tissue
Stimulation
Breast Neoplasms
Tumor initiation
Biology
03 medical and health sciences
0302 clinical medicine
Breast cancer
Western blot
BRCA1 mutation
Cell Movement
Cell Line, Tumor
medicine
Biomarkers, Tumor
Humans
skin and connective tissue diseases
Adipose-derived stromal cells
Migration
Cell Proliferation
Lipofilling
BRCA2 Protein
Original Paper
medicine.diagnostic_test
BRCA1 Protein
Mesenchymal Stem Cells
medicine.disease
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Culture Media, Conditioned
Cancer cell
Mutation
Cancer research
Female
Subjects
Details
- Language :
- English
- ISSN :
- 15737039 and 10833021
- Volume :
- 26
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Mammary Gland Biology and Neoplasia
- Accession number :
- edsair.doi.dedup.....df4729f612d220b20170aab25a59c682