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Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens
- Source :
- PLoS ONE, Vol 16, Iss 11, p e0259165 (2021), PLoS ONE, 16:e0259165. Public Library of Science, PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry based (MS) proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values
- Subjects :
- RNA viruses
Proteomics
Viral Diseases
Proteome
Coronaviruses
Physiology
Proteomes
viruses
Biochemistry
Nucleocapsids
Mass Spectrometry
Analytical Chemistry
Medical Conditions
Spectrum Analysis Techniques
Liquid chromatography–mass spectrometry
Chlorocebus aethiops
Pathology and laboratory medicine
Virus Testing
Liquid Chromatography
Multidisciplinary
Chromatographic Techniques
virus diseases
Medical microbiology
Body Fluids
Chemistry
Targeted proteomics
Infectious Diseases
Viruses
Physical Sciences
Medicine
SARS CoV 2
Pathogens
Anatomy
medicine.symptom
Research Article
SARS coronavirus
Coronavirus disease 2019 (COVID-19)
Liquid Chromatography-Mass Spectrometry
Science
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Viral Structure
Biology
Research and Analysis Methods
Mass spectrometry
Microbiology
Viral Proteins
Diagnostic Medicine
Virology
medicine
Animals
Humans
Nucleocapsid
Vero Cells
Medicine and health sciences
Detection limit
Biology and life sciences
SARS-CoV-2
Organisms
Viral pathogens
Sputum
Proteins
COVID-19
Covid 19
Phosphoproteins
Molecular biology
Microbial pathogens
Mucus
Targeted mass spectrometry
Vero cell
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....df446f11983bad5a58a68ee4176c55a7