Back to Search
Start Over
Pun1p is a metal ion-inducible, calcineurin/Crz1p-regulated plasma membrane protein required for cell wall integrity
- Source :
- Biochimica et Biophysica Acta
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Under conditions of environmental stress, the plasma membrane is involved in several regulatory processes to promote cell survival, like maintenance of signaling pathways, cell wall organization and intracellular ion homeostasis. PUN1 encodes a plasma membrane protein localizing to the ergosterol-rich membrane compartment occupied also by the arginine permease Can1. We found that the PUN1 (YLR414c) gene is transcriptionally induced upon metal ion stress. Northern blot analysis of the transcriptional regulation of PUN1 showed that the calcium dependent transcription factor Crz1p is required for PUN1 induction upon heavy metal stress. Here we report that mutants deleted for PUN1 exhibit increased metal ion sensitivity and morphological abnormalities. Microscopical and ultrastructural observations revealed a severe cell wall defect of pun1∆ mutants. By using chemical cross-linking, Blue native electrophoresis, and co-immunoprecipitation we found that Pun1p forms homo-oligomeric protein complexes. We propose that Pun1p is a stress-regulated factor required for cell wall integrity, thereby expanding the functional significance of lateral plasma membrane compartments.<br />Research highlights ► PUN1 is induced upon metal ion stress. ► Deletion of PUN1 causes metal ion sensitivity and a yeast cell wall defect. ► Under metal ion stress PUN1 is regulated by the calcineurin/Crz1p pathway. ► Pun1p forms higher-order protein complexes.
- Subjects :
- HOG, high osmolarity glycerol
Calcineurin/Crz1p
Biochemistry
Gene Expression Regulation, Fungal
Transcriptional regulation
Oligomerization
DIC, differential interference contrast
0303 health sciences
Calcineurin
Cell wall
TEM, transmission electron microscope
3. Good health
Cell biology
DNA-Binding Proteins
D, digitonin
Signal transduction
Intracellular
Plasma membrane
Vesicle-associated membrane protein 8
Saccharomyces cerevisiae Proteins
Green Fluorescent Proteins
Immunoblotting
Biophysics
Saccharomyces cerevisiae
Biology
DM, n-Dodecyl-β-d-maltoside
ICP-MS, inductively coupled plasma mass spectrometry
Article
03 medical and health sciences
Metals, Heavy
Transcription factor
030304 developmental biology
Ions
030306 microbiology
Cell Membrane
Membrane Proteins
Cell Biology
MCC, membrane compartment occupied by Can1
Blotting, Northern
Microscopy, Electron
Ion homeostasis
Microscopy, Fluorescence
Metal ion stress
Cell wall organization
Mutation
o-PDM, o-phenylenedimaleimide
Co-IP, Co-Immunoprecipitation
Gene Deletion
TX, Triton X-100
Transcription Factors
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 1808
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....dee0ef4559e5bc9d9559d77eb92f4d66
- Full Text :
- https://doi.org/10.1016/j.bbamem.2011.01.002