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Image-Guided Fluorescence Endomicroscopy: From Macro- to Micro-Imaging of Radiation-Induced Pulmonary Fibrosis
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- Radiation-induced pulmonary fibrosis (RIPF) is a debilitating side effect of radiation therapy (RT) of several cancers including lung and breast cancers. Current clinical methods to assess and monitor RIPF involve diagnostic computed tomography (CT) imaging, which is restricted to anatomical macroscopic changes. Confocal laser endomicroscopy (CLE) or fluorescence endomicroscopy (FE) in combination with a fibrosis-targeted fluorescent probe allows to visualize RIPF in real-time at the microscopic level. However, a major limitation of FE imaging is the lack of anatomical localization of the endomicroscope within the lung. In this work, we proposed and validated the use of x-ray fluoroscopy-guidance in a rat model of RIPF to pinpoint the location of the endomicroscope during FE imaging and map it back to its anatomical location in the corresponding CT image. For varying endomicroscope positions, we observed a positive correlation between CT and FE imaging as indicated by the significant association between increased lung density on CT and the presence of fluorescent fiber structures with FE in RT cases compared to Control. Combining multimodality imaging allows visualization and quantification of molecular processes at specific locations within the injured lung. The proposed image-guided FE method can be extended to other disease models and is amenable to clinical translation for assessing and monitoring fibrotic damage.
- Subjects :
- 0301 basic medicine
Pulmonary Fibrosis
medicine.medical_treatment
lcsh:Medicine
Radiation induced
Article
Fluorescence
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Pulmonary fibrosis
Endomicroscopy
Animals
Medicine
Radiation Injuries
lcsh:Science
Lung
Multidisciplinary
Anatomical location
business.industry
lcsh:R
Endoscopy
medicine.disease
Rats
3. Good health
Radiation therapy
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
lcsh:Q
Tomography
Tomography, X-Ray Computed
business
Nuclear medicine
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ded97cd6f89c66fc5774d184b38ef0a2