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Basic fibroblast growth factor modulates synaptic transmission in cultured rat hippocampal neurons
- Source :
- Brain Research. 723:190-195
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Basic fibroblast growth factor (bFGF) is one of the effective growth factors that protect neurons against excitotoxic/ischemic injury and promote neuronal survival. In the present study, we examined the acute modulative effect of bFGF on synaptic transmission by monitoring spontaneous intracellular Ca 2+ ([Ca 2+ ]i]) oscillation, the amplitudes of which reflect excitatory and inhibitory inputs. The hippocampal cells from embryonic day 18 rats were cultured for 11–14 days, and changes in [Ca 2+ ]i of single neurons were measured by a microfluometrical technique with fura-2. The amplitude of spontaneous oscillation was decreased by 10 ng/ml bFGF, but not by nerve growth factor (10–1000 ng/ml). Acidic FGF (1000 ng/ml) had a weaker depressant effect. The effect of bFGF was counteracted by suramin. bFGF did not affect the increase in [Ca 2+ ]i evoked by glutamate agonists, NMDA or kainate, indicating that glutamate receptors are not involved in the mechanism. This is supported by similar results that kainate-evoked current was not affected by bFGF. On the other hand, bicuculline masked the effect of bFGF on the Ca 2+ oscillation. But GABA-evoked current was slightly decreased by bFGF. These results suggest the possible role of bFGF in modulating GABAergic rather than glutamatergic neurotransmission.
- Subjects :
- medicine.medical_specialty
N-Methylaspartate
Basic fibroblast growth factor
Kainate receptor
Suramin
Neurotransmission
Biology
Hippocampus
Synaptic Transmission
chemistry.chemical_compound
Glutamatergic
Internal medicine
medicine
Animals
Rats, Wistar
Molecular Biology
Cells, Cultured
Dose-Response Relationship, Drug
General Neuroscience
Glutamate receptor
Bicuculline
Rats
Nerve growth factor
Endocrinology
chemistry
NMDA receptor
Fibroblast Growth Factor 2
Neurology (clinical)
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 723
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....ded1e814dce5f812a0b56384b93050ca