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An overview of techniques for immobilizing and viewing living cells

Authors :
Karl J. Aufderheide
Source :
Micron. 39:71-76
Publication Year :
2008
Publisher :
Elsevier BV, 2008.

Abstract

Microscopists making observations on living cells are often faced with the challenge of getting those cells to hold still for extended observation times. This paper presents an overview/summary of a range of techniques for non-destructive immobilization of living cells (with an emphasis on protozoa), permitting microscopic observations and photography. A variety of chemical and physical immobilization techniques are discussed, but particular attention is paid to a comparative discussion of the mechanical devices (rotocompressors or microcompressors) used for reversible trapping of living cells.

Details

ISSN :
09684328
Volume :
39
Database :
OpenAIRE
Journal :
Micron
Accession number :
edsair.doi.dedup.....deca292830618b4ced278b891fe0ce6d
Full Text :
https://doi.org/10.1016/j.micron.2006.12.002