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In Vitro Model using Mouse Hepatocytes for Study of Alcohol Stress
- Source :
- Bioscience, Biotechnology, and Biochemistry. 65:1528-1533
- Publication Year :
- 2001
- Publisher :
- Oxford University Press (OUP), 2001.
-
Abstract
- In this study, the effects of ethanol and allyl alcohol on primary mouse hepatocytes were investigated. No cytotoxicity was observed by ethanol treatments, but more toxicity to cells was found in the response to allyl alcohol treatment. The expression of cytochrome P450 2E1 (CYP2E1), phase I enzyme was examined in response to ethanol and allyl alcohol. Both xenobiotics induced CYP2E1 up to 1.5 to approximately 5 fold at the protein level. The effects of insulin on CYP2E1 expression were also measured. Insulin, which has been regarded as an essential hormone for primary hepatocytes, was shown to decrease the level of CYP2E1 protein, and did not affect cell viability. These results on CYP2E1 induction demonstrate that primary mouse hepatocytes, when using ethanol and allyl alcohol as substrates and in insulin-free medium, provide a suitable system for the studies of the role of CYP2E1 in xenobiotic metabolism and toxicity.
- Subjects :
- Cell Survival
Propanols
Alcohol
In Vitro Techniques
Models, Biological
Applied Microbiology and Biotechnology
Biochemistry
Xenobiotics
Analytical Chemistry
Mice
chemistry.chemical_compound
Ammonia
medicine
Animals
Insulin
Viability assay
Allyl alcohol
Molecular Biology
Mice, Inbred ICR
Ethanol
Dose-Response Relationship, Drug
organic chemicals
Organic Chemistry
Cytochrome P-450 CYP2E1
General Medicine
CYP2E1
medicine.anatomical_structure
chemistry
Hepatocyte
Toxicity
Hepatocytes
Drug metabolism
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....dec84b342bdd70699720800a6334c0f1