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Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage
- Source :
- Journal of Molecular Cell Biology
- Publication Year :
- 2019
-
Abstract
- Chemotherapeutic agents are extensively used to treat malignancies. However, chemotherapy-induced ovarian damage and reduced fertility are severe side effects. Recently, stem cell transplantation has been reported to be an effective strategy for premature ovarian insufficiency (POI) treatment, but safety can still be an issue in stem cell-based therapy. Here, we show the protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium (hUCMSC-CM) on a cisplatin (Cs)-induced ovarian injury model. hUCMSC-CM can relieve Cs-induced depletion of follicles and preserve fertility. In addition, hUCMSC-CM can decrease apoptosis of oocytes and granulosa cells induced by Cs. RNA sequencing analysis reveals the differentially expressed genes of ovaries after Cs and hUCMSC-CM treatments, including genes involved in cell apoptosis. Furthermore, we show that the granulocyte colony-stimulating factor (G-CSF)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway plays an important role in protecting granulosa cells from Cs-induced apoptosis. Together, we confirm the protective effects of hUCMSC-CM on ovarian reserve and fertility in mice treated with Cs, highlighting the remarkable therapeutic effects of hUCMSC-CM.
- Subjects :
- 0301 basic medicine
Apoptosis
AcademicSubjects/SCI01180
Umbilical Cord
Mice
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Ovarian Follicle
Granulocyte Colony-Stimulating Factor
ovarian damage
Ovarian Reserve
Cells, Cultured
RNA sequencing
General Medicine
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
Stem cell
Signal Transduction
Down-Regulation
Biology
Premature ovarian insufficiency
Mesenchymal Stem Cell Transplantation
Protective Agents
Article
03 medical and health sciences
Neutralization Tests
Genetics
medicine
Animals
Humans
Ovarian follicle
Ovarian reserve
Molecular Biology
Protein kinase B
PI3K/AKT/mTOR pathway
Granulosa Cells
Mesenchymal stem cell
Ovary
Reproducibility of Results
Mesenchymal Stem Cells
Cell Biology
Transplantation
stem cell
030104 developmental biology
Fertility
Culture Media, Conditioned
Cancer research
Cisplatin
Transcriptome
Proto-Oncogene Proteins c-akt
Subjects
Details
- ISSN :
- 17594685
- Volume :
- 12
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of molecular cell biology
- Accession number :
- edsair.doi.dedup.....dea1b73151e8f88b480ca3e71cb9d685