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Depression of Fungal Polygalacturonase Activity in Solanum lycopersicum Contributes to Antagonistic Yeast-Mediated Fruit Immunity to Botrytis
- Source :
- Journal of agricultural and food chemistry. 67(12)
- Publication Year :
- 2019
-
Abstract
- The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.
- Subjects :
- 0106 biological sciences
food.ingredient
Cell wall disassembly
Polygalacturonase activity
01 natural sciences
Microbiology
Fungal Proteins
food
Solanum lycopersicum
Gene Expression Regulation, Plant
Yeasts
Antibiosis
Pectinase
Botrytis cinerea
Botrytis
Disease Resistance
Plant Proteins
biology
fungi
010401 analytical chemistry
food and beverages
Ripening
General Chemistry
biology.organism_classification
0104 chemical sciences
Polygalacturonase
Fruit
Pectins
Polygalacturonase inhibitor
Solanum
General Agricultural and Biological Sciences
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15205118
- Volume :
- 67
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of agricultural and food chemistry
- Accession number :
- edsair.doi.dedup.....de30f2e09667385f417c57ead9222040