Back to Search
Start Over
Role of inducible nitric oxide synthase in the regulation of VCAM-1 expression in gut inflammation
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 277:G572-G576
- Publication Year :
- 1999
- Publisher :
- American Physiological Society, 1999.
-
Abstract
- The objectives of this study were to assess the role of the inducible isoform of nitric oxide synthase (iNOS) on vascular cell adhesion molecule 1 (VCAM-1) expression in vivo in an acute model of inflammation induced in iNOS-deficient (iNOS−/−) mice and compare these data to those obtained by pharmacological inhibition of iNOS in a CD4+ T lymphocyte-dependent model of chronic colitis. VCAM-1 expression was quantified in vivo using the dual radiolabel monoclonal antibody technique. We found that intraperitoneal injection of 10 μg/kg tumor necrosis factor-α (TNF-α) enhanced VCAM-1 expression by approximately twofold in the colon, cecum, and stomach but not small intestine in iNOS−/−mice compared with TNF-α-injected wild-type mice. Injection of wild-type mice with 25 μg/kg TNF-α further enhanced VCAM-1 expression by approximately twofold compared with wild-type mice injected with 10 μg/kg TNF-α; however, VCAM-1 expression was not further enhanced in any gastrointestinal organ system in iNOS−/− mice. In a second series of experiments, we found that continuous inhibition of iNOS using oral administration of N G-iminoethyl-l-lysine did not alter the enhanced levels of VCAM-1 expression in the colon nor did it alter the severity of colonic inflammation in SCID mice reconstituted with CD4+, CD45RBhigh T cells. We conclude that iNOS may regulate VCAM-1 expression in acute inflammation; however, this effect is modest and tissue specific and occurs only when VCAM-1 expression is submaximal. iNOS does not appear to modulate VCAM-1 expression in an immune model of chronic colitis.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
medicine.medical_specialty
Physiology
T-Lymphocytes
medicine.medical_treatment
Intraperitoneal injection
Nitric Oxide Synthase Type II
Vascular Cell Adhesion Molecule-1
Mice, SCID
Biology
Nitric oxide
Mice
Cecum
chemistry.chemical_compound
In vivo
Physiology (medical)
Internal medicine
medicine
Animals
VCAM-1
Mice, Knockout
Hepatology
Tumor Necrosis Factor-alpha
Gastroenterology
Colitis
Molecular biology
Recombinant Proteins
Small intestine
Mice, Inbred C57BL
Nitric oxide synthase
medicine.anatomical_structure
Endocrinology
chemistry
Chronic Disease
biology.protein
Leukocyte Common Antigens
Tumor necrosis factor alpha
Nitric Oxide Synthase
Injections, Intraperitoneal
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 277
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....de2e465faf9e628583aa11a1b1710aa1