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Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
- Source :
- Vaccine. 34:5209-5216
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In the present study, we developed IBV S1 glycoprotein poly-epitope-based DNA vaccine pV-S1B+S1T consisting of SH1208 and Holte strain BF2-restricted T cell epitopes and Australian T strain dominant B cell neutralization epitopes. Specific pathogen-free chickens were vaccinated with pV-S1B+S1T and control plasmids twice to elicit strong humoral and cellular immune response, as indicated by viral neutralization titers and results of CD8+ T cell proliferation assays. A lethal dose of IBV SH1208 strain used for protection and challenge experiments at two weeks post-booster immunization following challenge protection and virus shedding reverse transcription quantitative PCR assay, indicated that pV-S1B+S1T protected against IBV and significantly reduced viral excretion. These results demonstrated that the IBV poly-epitope-based vaccine effectively prevents infection and represents a potential IBV vaccine.
- Subjects :
- 0301 basic medicine
Cellular immunity
animal structures
040301 veterinary sciences
Infectious bronchitis virus
Immunization, Secondary
Epitopes, T-Lymphocyte
CD8-Positive T-Lymphocytes
Biology
Lymphocyte Activation
Epitope
Neutralization
Microbiology
DNA vaccination
0403 veterinary science
03 medical and health sciences
Immune system
Animals
Viral shedding
Poultry Diseases
Immunity, Cellular
General Veterinary
General Immunology and Microbiology
Public Health, Environmental and Occupational Health
Viral Vaccines
04 agricultural and veterinary sciences
Antibodies, Neutralizing
Virology
Immunity, Humoral
Specific Pathogen-Free Organisms
Virus Shedding
030104 developmental biology
Infectious Diseases
Immunization
Acute Disease
Spike Glycoprotein, Coronavirus
embryonic structures
Molecular Medicine
Coronavirus Infections
Chickens
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....de2d7100934b5e0376154f36cbe64a21