Back to Search
Start Over
A New Enterobacter cloacae Bacteriophage EC151 Encodes the Deazaguanine DNA Modification Pathway and Represents a New Genus within the Siphoviridae Family
- Source :
- Viruses, Vol 13, Iss 1372, p 1372 (2021), Viruses, Volume 13, Issue 7
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 genome is 60,753 bp and contains 58 putative genes. Thirty-nine of them encode proteins of predicted function, 18 are defined as hypothetical proteins, and one ORF identifies as the tRNA-Ser-GCT-encoding gene. Six ORFs were predicted to be members of the deazaguanine DNA modification pathway, including the preQ0 transporter. Comparative proteomic phylogenetic analysis revealed that phage EC151 represents a distinct branch within a group of sequences containing clades formed by members of the Seuratvirus, Nonagvirus, and Vidquintavirus genera. In addition, the EC151 genome showed gene synteny typical of the Seuratvirus, Nonagvirus, and Nipunavirus phages. The average genetic distances of EC151/Seuratvirus, EC151/Nonagvirus, and EC151/Vidquintavirus are approximately equal to those between the Seuratvirus, Nonagvirus, and Vidquintavirus genera (~0.7 substitutions per site). Therefore, EC151 may represent a novel genus within the Siphoviridae family. The origin of the deazaguanine DNA modification pathway in the EC151 genome can be traced to Escherichia phages from the Seuratvirus genus.
- Subjects :
- Proteomics
Enterobacter phage
Genomics
Genome, Viral
Siphoviridae
Biology
Genome
Microbiology
Host Specificity
Article
Bacteriophage
Viral Proteins
03 medical and health sciences
Nonagvirus
Seuratvirus
Virology
Enterobacter cloacae
Bacteriophages
Gene
Phylogeny
030304 developmental biology
Synteny
Genetics
0303 health sciences
030306 microbiology
Virion
Nucleic acid sequence
preQ0 transporter
biology.organism_classification
QR1-502
deazaguanine modification pathway
Infectious Diseases
GenBank
DNA, Viral
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 13
- Issue :
- 1372
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....de1b5d08db3e4cf8fc6a81c205fbeab0