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Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
- Publication Year :
- 2018
- Publisher :
- Cold Spring Harbor Laboratory Press, 2018.
-
Abstract
- MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA–RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.
- Subjects :
- 0301 basic medicine
Messenger RNA
Trypanosoma
RNA, Mitochondrial
Protozoan Proteins
RNA
RNA-Binding Proteins
RNA-binding protein
Plasma protein binding
Biology
Mitochondrion
In vitro
Article
Cell biology
Mitochondria
03 medical and health sciences
030104 developmental biology
RNA editing
RNA Editing
RNA, Messenger
Molecular Biology
ICLIP
Protein Binding
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....de17003548b691ae8b7b8b4be561f9cf