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Characterization of β-adrenergic receptors of freshly isolated astrocytes and neurons from rat brain
- Source :
- Life Sciences. 60:315-324
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- The binding and characteristics of rat brain beta-adrenergic receptors (beta-AR) isolated from astrocytes and neurons were investigated. Equilibrium binding experiments demonstrated that beta-AR were more concentrated on astrocytes than on neurons isolated from forebrain, cerebral cortex and cerebellum. Inhibition experiments revealed that beta 1-AR and beta 2-AR were present in the two cell types. Isoproterenol revealed two interchangeable states of high and low affinity binding to both beta 1- and beta 2-AR in neurons. The high affinity binding sites were sensitive to guanylylimidodiphosphate (GppNHp). Similar results were found with other beta-AR agonists but not with salbutamol and salmeterol which recognized both affinity states of the neuronal beta 2-AR but only the low affinity state of beta 1-AR. In astrocytes only the low affinity state of beta-AR was observed.
- Subjects :
- Male
Cell type
Cerebellum
High affinity binding
Adrenergic beta-Antagonists
Biology
Binding, Competitive
General Biochemistry, Genetics and Molecular Biology
Propanolamines
Prosencephalon
Receptors, Adrenergic, beta
medicine
Animals
Rats, Wistar
General Pharmacology, Toxicology and Pharmaceutics
Receptor
Cells, Cultured
Neurons
Imidazoles
General Medicine
Adrenergic beta-Agonists
Rat brain
Immunohistochemistry
Rats
medicine.anatomical_structure
Cerebral cortex
Astrocytes
Forebrain
Biophysics
β adrenergic receptor
Neuroscience
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....de135f9ef50f84c9df8c407b46a0a001