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Flow-dependent epigenetic regulation of IGFBP5 expression by H3K27me3 contributes to endothelial anti-inflammatory effects
- Source :
- Theranostics
- Publication Year :
- 2018
- Publisher :
- Ivyspring International Publisher, 2018.
-
Abstract
- Rationale: Atherosclerosis is a chronic inflammatory and epigenetic disease that is influenced by different patterns of blood flow. However, the epigenetic mechanism whereby atheroprotective flow controls endothelial gene programming remains elusive. Here, we investigated the possibility that flow alters endothelial gene expression through epigenetic mechanisms. Methods: En face staining and western blot were used to detect protein expression. Real-time PCR was used to determine relative gene expression. RNA-sequencing of human umbilical vein endothelial cells treated with siRNA of enhancer of zeste homolog 2 (EZH2) or laminar flow was used for transcriptional profiling. Results: We found that trimethylation of histone 3 lysine 27 (H3K27me3), a repressive epigenetic mark that orchestrates gene repression, was reduced in laminar flow areas of mouse aorta and flow-treated human endothelial cells. The decrease of H3K27me3 paralleled a reduction in the epigenetic “writer”-EZH2, the catalytic subunit of the polycomb repressive complex 2 (PRC2). Moreover, laminar flow decreased expression of EZH2 via mechanosensitive miR101. Genome-wide transcriptome profiling studies in endothelial cells treated with EZH2 siRNA and flow revealed the upregulation of novel mechanosensitive gene IGFBP5 (insulin-like growth factor-binding protein 5), which is epigenetically silenced by H3K27me3. Functionally, inhibition of H3K27me3 by EZH2 siRNA or GSK126 (a specific EZH2 inhibitor) reduced H3K27me3 levels and monocyte adhesion to endothelial cells. Adenoviral overexpression of IGFBP5 also recapitulated the anti-inflammatory effects of H3K27me3 inhibition. More importantly, we observed EZH2 upregulation, and IGFBP5 downregulation, in advanced atherosclerotic plaques from human patients. Conclusion: Taken together, our findings reveal that atheroprotective flow reduces H3K27me3 as a chromatin-based mechanism to augment the expression of genes that confer an anti-inflammatory response in the endothelium. Our study exemplifies flow-dependent epigenetic regulation of endothelial gene expression, and also suggests that targeting the EZH2/H3K27me3/IGFBP5 pathway may offer novel therapeutics for inflammatory disorders such as atherosclerosis.
- Subjects :
- 0301 basic medicine
Endothelium
H3K27me3
Anti-Inflammatory Agents
Medicine (miscellaneous)
macromolecular substances
Methylation
Epigenesis, Genetic
Histones
Mice
03 medical and health sciences
Downregulation and upregulation
Gene expression
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Enhancer of Zeste Homolog 2 Protein
EZH2
Epigenetics
RNA, Small Interfering
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
biology
Polycomb Repressive Complex 2
Genetic Therapy
Atherosclerosis
endothelial cells
3. Good health
Chromatin
Cell biology
Gene Expression Regulation, Neoplastic
030104 developmental biology
Histone
medicine.anatomical_structure
biology.protein
IGFBP5
Insulin-Like Growth Factor Binding Protein 5
PRC2
epigenetic
Research Paper
Subjects
Details
- ISSN :
- 18387640
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Theranostics
- Accession number :
- edsair.doi.dedup.....ddfca071b20fe11ceb90824cdb5c5d61