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The impact of CUP1 gene copy-number and XVI-VIII/XV-XVI translocations on copper and sulfite tolerance in vineyard Saccharomyces cerevisiae strain populations
- Source :
- FEMS Yeast Research. 20
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- In wine production, sulfites are widely used as antimicrobials and antioxidants, whereas copper is associated with fungicides and wine fining treatments. Therefore, wine yeasts are constantly exposed to these agents. Copper tolerance is related to the copy number of the CUP1 gene, encoding for a metallothionein involved in copper detoxification. In wine yeasts, sulfite resistance mainly depends on the presence of the translocation t(XVI;VIII) in the promoter region of the SSU1 gene. This gene encodes for a plasma membrane sulfite pump involved in sulfite metabolism and detoxification. Recently, a new translocation, t(XVI;VIII), was identified. In this work, 253 Saccharomyces cerevisiae strains, representing three vineyard populations from two different continents, were analyzed, along with 20 industrial starters. Copper and sulfites tolerance as well as distribution of CUP1 gene copy-number, t(XVI;VIII)and t(XVI;XV) of SSU1 gene were studied to evaluate the impact of these genomic variations on population phenotypes. The CUP1 gene copy-number was found to be highly variable, ranging from zero to 79 per strain. Moreover it differently impacted the copper tolerance in the populations of the two continents. The diffusion of t(XVI;VIII) and, for the first time, t(XVI;XV) was determined in the three vineyard populations. The correlation between the presence of the translocation and strain sulfite tolerance levels was significant only for the t(XVI;VIII).
- Subjects :
- Saccharomyces cerevisiae Proteins
Anion Transport Proteins
Population
Saccharomyces cerevisiae
Gene Dosage
chromosomal translocation
copper resistance
copy number variation (CNV)
sulfites resistance
wine yeast
Wine
Chromosomal translocation
Applied Microbiology and Biotechnology
Microbiology
Translocation, Genetic
chemistry.chemical_compound
Sulfite
Sulfites
Metallothionein
education
Gene
education.field_of_study
biology
General Medicine
biology.organism_classification
Yeast in winemaking
chemistry
Biochemistry
Fermentation
Copper
Subjects
Details
- ISSN :
- 15671364 and 15671356
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- FEMS Yeast Research
- Accession number :
- edsair.doi.dedup.....ddf42b9020174dd7c036dea8ab4db8c5