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<scp>KITLG</scp> is a novel target of miR‐34c that is associated with the inhibition of growth and invasion in colorectal cancer cells
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- MiR-34c is considered a potent tumour suppressor because of its negative regulation of multiple target mRNAs that are critically associated with tumorigenesis and metastasis. In the present study, we demonstrated a novel target of miR-34c, KITLG, which has been implicated in colorectal cancer (CRC). First, we found a significant negative relationship between miR-34c and KITLG mRNA expression levels in CRC cell lines, including HT-29, HCT-116, SW480 and SW620 CRC cell lines. In silico analysis predicted putative binding sites for miR-34c in the 3' untranslated region (3'UTR) of KITLG mRNA. A dual-luciferase reporter assay further confirmed that KITLG is a direct target of miR-34c. Then, the cell lines were infected with lentiviruses expressing miR-34c or a miR-34c specific inhibitor. Restoration of miR-34c dramatically reduced the expression of KITLG mRNA and protein, while silencing of endogenous miR-34c increased the expression of KITLG protein. The miR-34c-mediated down-regulation of KITLG was associated with the suppression on proliferation, cellular transformation, migration and invasion of CRC cells, as well as the promotion on apoptosis. Knockdown of KITLG by its specific siRNA confirmed a critical role of KITLG down-regulation for the tumour-suppressive effects of miR-34c in CRC cells. In conclusion, our results demonstrated that miR-34c might interfere with KITLG-related CRC and could be a novel molecular target for CRC patients.
- Subjects :
- Untranslated region
colorectal cancer cell
tumour suppressor
Blotting, Western
Fluorescent Antibody Technique
Apoptosis
Biology
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Cell Movement
microRNA
Tumor Cells, Cultured
medicine
Humans
Gene silencing
RNA, Messenger
RNA, Small Interfering
Cell Proliferation
Stem Cell Factor
Gene knockdown
Reporter gene
Reverse Transcriptase Polymerase Chain Reaction
Cell growth
Cell Cycle
Original Articles
Cell Biology
Cell cycle
Molecular biology
Gene Expression Regulation, Neoplastic
MicroRNAs
Cancer research
Molecular Medicine
KITLG
miR-34c
Colorectal Neoplasms
Carcinogenesis
Subjects
Details
- ISSN :
- 15824934 and 15821838
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....ddd6c7118762f4ff28c72457e5857547