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Biological and immunological characterization of influenza virus haemagglutinin expressed from the haemagglutinin locus of vaccinia virus
- Source :
- Journal of General Virology. 71:1293-1301
- Publication Year :
- 1990
- Publisher :
- Microbiology Society, 1990.
-
Abstract
- Plasmid vectors were constructed to facilitate the insertion and expression of a foreign gene in the haemagglutinin (HA) gene locus of vaccinia virus. Five unique cloning sites adjacent to the P7.5 promoter of vaccinia virus permit the rapid insertion of a foreign sequence coding for a protein into these plasmids. This vector system provides a simple procedure to select recombinant viruses because they can be readily identified on the basis of their HA-defective phenotype. Recombinant vaccinia viruses expressing influenza virus HA were constructed to characterize the possible use of this system. The recombinant viruses did express the influenza HA through the authentic pathway of biosynthesis. In addition to having immunological characteristics similar to the authentic influenza HA, the expressed HA was found to possess haemagglutinating, haemadsorption and acid-inducible fusion activities. These findings demonstrate the usefulness of this eukaryotic vector system.
- Subjects :
- viruses
Genetic Vectors
Gene Expression
Hemagglutinins, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Vaccinia virus
Locus (genetics)
Biology
Membrane Fusion
Virus
Cell Line
law.invention
chemistry.chemical_compound
Plasmid
Biosynthesis
law
Virology
Animals
Hemadsorption
Antigens, Viral
Gene
Hemagglutination, Viral
Hemagglutination Inhibition Tests
Phenotype
chemistry
Influenza A virus
Recombinant DNA
Vaccinia
Plasmids
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....ddb5792df7208e4b8e4f720328dccb68